{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:38:41Z","timestamp":1770824321466,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T00:00:00Z","timestamp":1547510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008061","name":"Fundaci\u00f3n Mutua Madrile\u00f1a","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008061","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>(1) To identify p16 protein in laryngeal squamous cell carcinoma (LSCC) specimens and to correlate it with the presence of human papillomavirus (HPV) found in these specimens from a previous study. (2) To analyze p16 impact on 10\u2010year overall and disease\u2010free survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Retrospective case series with oncologic database chart review.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Academic tertiary care hospital.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects<\/jats:title><jats:p>A total of 123 samples of LSCC (taken from the glottis only) from patients treated with primary surgical resection between 1977 and 2005.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>p16 protein expression was analyzed through immunohistochemistry and compared with the presence of HPV established in our previous studies. Results were compared with histologic, clinicopathologic, and survival parameters, with a 10\u2010year follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the samples, 39.02% were positive for p16, but only 11.38% were positive for both p16 and HPV. The p16+ cohort showed a significant improvement in disease\u2010free survival (<jats:italic>P<\/jats:italic> =. 0022); statistical significance was not achieved for overall survival. p16+ cases had fewer relapses over time, with no relapses after a 2\u2010year follow\u2010up. Age at the time of diagnosis and tobacco consumption were the only epidemiologic factors that influenced overall survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The expression of p16 protein was a beneficial prognostic factor for disease\u2010free survival among patients with LSCC of the glottis, with no relapses after a 2\u2010year follow\u2010up.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599818821910","type":"journal-article","created":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T04:54:26Z","timestamp":1547528066000},"page":"1042-1047","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["p16 Influence on Laryngeal Squamous Cell Carcinoma Relapse and Survival"],"prefix":"10.1002","volume":"160","author":[{"given":"Alvaro","family":"S\u00e1nchez Barrueco","sequence":"first","affiliation":[{"name":"ENT and Cervicofacial Surgery Department Hospital Universitario Fundaci\u00f3n 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