{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T23:56:50Z","timestamp":1780617410954,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010836","name":"American Laryngological, Rhinological and Otological Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY020853"],"award-info":[{"award-number":["EY020853"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY014800"],"award-info":[{"award-number":["EY014800"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The goal of the study was to determine whether reactive oxygen species (ROS) mediates cytomegalovirus (CMV)\u2013induced labyrinthitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Murine model of CMV infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>University of Utah laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Nrf2\u00a0knockout mice were inoculated with murine CMV. Auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAEs) were then performed on these and uninfected controls. BALB\/c mice were inoculated with murine CMV to determine whether a marker for ROS production, dihydroethidium (DHE), is expressed 7\u00a0days after inoculation. Finally, 2 antioxidants\u2014D\u2010methionine and ACE\u2010Mg (vitamins A, C, and E with magnesium)\u2014were administered 1 hour before and after infection in inoculated mice for 14\u00a0days. Temporal bones were harvested at postnatal day 10 for DHE detection. ABR and DPOAE testing was done at postnatal day 30. Scanning electron microscopy was also performed at postnatal day 30 to evaluate outer hair cell integrity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nrf2\u2010infected mice had worse hearing than uninfected mice (<jats:italic>P<\/jats:italic> &lt;. 001). A statistically significant increase in DHE fluorescence was detected in BALB\/c\u2010infected mice as compared with uninfected mice 7\u00a0days after inoculation. D\u2010methionine\u2010 and ACE\u2010Mg\u2010treated mice demonstrated an attenuation of the DHE fluorescence and a significant improvement in ABR and DPOAE thresholds when compared with untreated infected controls (<jats:italic>P<\/jats:italic> &lt;. 0001). Scanning electron microscopy demonstrated less outer hair cell loss in the treated versus untreated infected controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results demonstrate for the first time that excessive ROS mediates CMV\u2010induced hearing loss in a mouse model.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599820901485","type":"journal-article","created":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T04:48:15Z","timestamp":1581396495000},"page":"709-717","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Role of Free Radical Formation in Murine Cytomegalovirus\u2013Induced Hearing Loss"],"prefix":"10.1002","volume":"162","author":[{"given":"Phayvanh P.","family":"Pecha","sequence":"first","affiliation":[{"name":"Division of Pediatric Otolaryngology Medical University of South Carolina Charleston South Carolina 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