{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:32:36Z","timestamp":1761006756128,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T00:00:00Z","timestamp":1589846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008308","name":"Comprehensive Cancer Center, City of Hope","doi-asserted-by":"publisher","award":["NCI P30 CA16058"],"award-info":[{"award-number":["NCI P30 CA16058"]}],"id":[{"id":"10.13039\/100008308","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To examine the microRNA (miRNA) expression profile of cutaneous squamous cell carcinoma (cSCC) tumors from aggressive head and neck locations compared with nonaggressive anatomic sites and normal controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Retrospective analysis of miRNA expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Tertiary care center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Tissue samples were collected from 3 anatomic regions: aggressive head and neck sites (ie, ears\/lip), nonaggressive locations (ie, extremities\/trunk), and adjacent normal skin. RNA was isolated from tissue cores of 45 samples (18 aggressive sites, 15\u00a0nonaggressive sites, and 12\u00a0normal\u2010adjacent skin). miRNA expression analysis was completed for approximately 800\u00a0miRNAs using the NanoString nCounter panel. Five candidate miRNAs were selected for validation. Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) was performed on the original samples plus 30 additional tissue samples (7 aggressive sites, 14\u00a0nonaggressive sites, and 9\u00a0normal\u2010adjacent skin).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five candidate miRNAs with significant differences in miRNA expression (<jats:italic>P<\/jats:italic> &lt; 0 \u2264. 001) from discovery samples were selected: <jats:italic>miR\u201021, miR\u201031, let\u20107g, miR\u201093<\/jats:italic>, and <jats:italic>miR\u201022<\/jats:italic>. Relative expression for these miRNAs using qRT\u2010PCR in the new sample set did not reveal any significant differences using 1\u2010way analysis of variance. When sets were combined, <jats:italic>miR\u201021<\/jats:italic> showed increased expression in aggressive tumors relative to nonaggressive tumors (<jats:italic>P<\/jats:italic> =. 009), but no others reached statistical significance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>cSCC behaves more aggressively when originating from specific anatomical subsites of the head and neck. Of 5\u00a0miRNAs evaluated, only <jats:italic>miR\u201021<\/jats:italic> showed significantly higher expression in tumors from aggressive sites relative to nonaggressive sites. Larger sample sizes are needed to evaluate other miRNAs.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599820918855","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T04:01:54Z","timestamp":1589860914000},"page":"538-545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["MicroRNA Expression Profiling of Cutaneous Squamous Cell Carcinomas Arising in Different Sites"],"prefix":"10.1002","volume":"163","author":[{"given":"Alexander N.","family":"Rock","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery The Ohio State University Wexner Medical Center Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mason D.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics Comprehensive Cancer Center The Ohio State University Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwenyth","family":"Amborski","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics Comprehensive Cancer Center The Ohio State University Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn C.","family":"Allain","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Division of Human Genetics The Ohio State University Wexner Medical Center Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"Klee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Division of Human Genetics The Ohio State University Wexner Medical Center Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara B.","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Pathology Division of Dermatopathology The Ohio State University Wexner Medical Center Columbus Ohio USA"},{"name":"Central Ohio Skin and Cancer Westerville Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery The Ohio State University Wexner Medical Center Columbus Ohio USA"},{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Arthur G. James Cancer Hospital and Richard J. Solove Research Institute The Ohio State University Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda E.","family":"Toland","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics Comprehensive Cancer Center The Ohio State University Columbus Ohio USA"},{"name":"Department of Internal Medicine Division of Human Genetics The Ohio State University Wexner Medical Center Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,19]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.1016\/j.jaad.2017.08.059"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_1","DOI":"10.1001\/jamadermatol.2015.4994"},{"key":"e_1_2_8_4_1","first-page":"231475","article-title":"Molecular markers in cutaneous squamous cell carcinoma","volume":"2011","author":"Tufaro AP","year":"2011","journal-title":"Int J Surg Oncol"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1016\/0190-9622(92)70144-5"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_1","DOI":"10.1002\/jso.23155"},{"doi-asserted-by":"publisher","key":"e_1_2_8_7_1","DOI":"10.1158\/1078-0432.CCR-18-1122"},{"doi-asserted-by":"publisher","key":"e_1_2_8_8_1","DOI":"10.1002\/emmm.201100209"},{"doi-asserted-by":"publisher","key":"e_1_2_8_9_1","DOI":"10.1038\/jid.2010.169"},{"doi-asserted-by":"publisher","key":"e_1_2_8_10_1","DOI":"10.1111\/jdv.13098"},{"doi-asserted-by":"publisher","key":"e_1_2_8_11_1","DOI":"10.1016\/j.jdermsci.2012.09.004"},{"doi-asserted-by":"publisher","key":"e_1_2_8_12_1","DOI":"10.1038\/nrg2634"},{"doi-asserted-by":"publisher","key":"e_1_2_8_13_1","DOI":"10.1111\/jcmm.12649"},{"doi-asserted-by":"publisher","key":"e_1_2_8_14_1","DOI":"10.1186\/1471-2407-12-505"},{"doi-asserted-by":"publisher","key":"e_1_2_8_15_1","DOI":"10.1038\/sj.onc.1210083"},{"doi-asserted-by":"publisher","key":"e_1_2_8_16_1","DOI":"10.1038\/sj.onc.1210856"},{"doi-asserted-by":"publisher","key":"e_1_2_8_17_1","DOI":"10.1074\/jbc.M707224200"},{"doi-asserted-by":"publisher","key":"e_1_2_8_18_1","DOI":"10.1158\/1078-0432.CCR-08-3053"},{"key":"e_1_2_8_19_1","first-page":"1625","article-title":"Expression of microRNAs in squamous cell carcinoma of human head and neck and the esophagus: miR\u2010205 and miR\u201021 are specific markers for HNSCC and ESCC","volume":"23","author":"Kimura S","year":"2010","journal-title":"Oncol Rep"},{"doi-asserted-by":"publisher","key":"e_1_2_8_20_1","DOI":"10.1007\/s00403-016-1705-0"},{"doi-asserted-by":"publisher","key":"e_1_2_8_21_1","DOI":"10.1371\/journal.pone.0103206"},{"doi-asserted-by":"publisher","key":"e_1_2_8_22_1","DOI":"10.1111\/exd.12153"},{"doi-asserted-by":"publisher","key":"e_1_2_8_23_1","DOI":"10.1016\/j.archoralbio.2016.02.013"},{"doi-asserted-by":"publisher","key":"e_1_2_8_24_1","DOI":"10.1186\/s12935-014-0138-0"},{"doi-asserted-by":"publisher","key":"e_1_2_8_25_1","DOI":"10.1007\/s13277-015-3038-6"},{"doi-asserted-by":"publisher","key":"e_1_2_8_26_1","DOI":"10.1371\/journal.pone.0102403"},{"doi-asserted-by":"publisher","key":"e_1_2_8_27_1","DOI":"10.1001\/jamadermatol.2018.0792"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599820918855","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1177\/0194599820918855","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599820918855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:09:16Z","timestamp":1760954956000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1177\/0194599820918855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,19]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1177\/0194599820918855"],"URL":"https:\/\/doi.org\/10.1177\/0194599820918855","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"type":"print","value":"0194-5998"},{"type":"electronic","value":"1097-6817"}],"subject":[],"published":{"date-parts":[[2020,5,19]]},"assertion":[{"value":"2019-10-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}