{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:26:56Z","timestamp":1761006416820,"version":"build-2065373602"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,5,6]],"date-time":"2016-05-06T00:00:00Z","timestamp":1462492800000},"content-version":"vor","delay-in-days":11297,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[1985,6]]},"abstract":"<jats:p>DNA originally extracted from squamous cell cancer of the larynx has been serially passaged through transformed populations of NIH\/3T3\u00a0mouse fibroblasts. The transformed foci were then harvested, cloned to volume, and incubated with a fresh population of NIH\/3T3 cells in a second passage. Transforming efficiencies were enhanced by serial passage. In addition, Southern Blot analysis of the transformed foci revealed hybridization between transformant DNA and human probe DNA from the Alu family of conserved human DNA sequences. In the first passage this hybridization took the form of diffuse homology throughout the entire molecular weight distribution. The second\u2010passage DNA showed \u201cnarrow bands\u201d indicating the possibility that an oncogene has been identified in laryngeal cancer and that serial passage has eliminated contaminating human sequences. Repetitive transfection in third\u2010 and fourth\u2010passage studies is now being completed.<\/jats:p>","DOI":"10.1177\/019459988509300311","type":"journal-article","created":{"date-parts":[[2017,7,13]],"date-time":"2017-07-13T14:54:08Z","timestamp":1499957648000},"page":"346-350","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Oncogenes in Laryngeal Cancer: Serial Passage of Transformed Cellular DNA"],"prefix":"10.1002","volume":"93","author":[{"given":"William H.","family":"Friedman","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery St. Louis University School of Medicine St. Louis Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry N.","family":"Rosenblum","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery St. Louis University School of Medicine St. Louis Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Loewenstein","sequence":"additional","affiliation":[{"name":"Institute for Molecular Virology St. Louis University School of Medicine St. Louis Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Thornton","sequence":"additional","affiliation":[{"name":"Institute for Molecular Virology St. Louis University School of Medicine St. Louis Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Katsantonis","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery St. Louis University School of Medicine St. Louis Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurice","family":"Green","sequence":"additional","affiliation":[{"name":"Institute for Molecular Virology St. Louis University School of Medicine St. Louis Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,6]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"801","article-title":"Cellular transforming genes","volume":"218","author":"Cooper GM","year":"1983","journal-title":"Science"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/58.6.1743"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90388-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/290261a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.11.5714"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.2.1181"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/302033a0"},{"issue":"10","key":"e_1_2_1_9_1","author":"Drug resistance","year":"1982","journal-title":"Oncology Times: The Newspaper for Specialists in Cancer"},{"key":"e_1_2_1_10_1","unstructured":"FriedmanWH RosenblumB LoewensteinP ThorntonH KatsantonisG GreenM:Cellular transforming genes in human head and neck surgery. 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