{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:43:22Z","timestamp":1761007402635,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T00:00:00Z","timestamp":1468886400000},"content-version":"vor","delay-in-days":10275,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[1988,6]]},"abstract":"<jats:p>Squamous cell carcinomas of the head and neck consist of heterogeneous cell populations. The purpose of the present study was to Investigate whether established cell lines from human head and neck cancers under chemotherapy behaved similarly to tumors in patients during in vivo treatment. This Is of Interest in terms of improvements of chemotherapeutic protocols and understanding of the mechanisms of cytotoxic drug resistance. Permanent squamous carcinoma cell lines of the larynx (HLaC 78, 79), parotid gland (HPaC 79), tongue (SCC\u201015, SCC\u201025), hypopharynx (FaDu), and tumor lines with different histology and origin, as mucoepidermoid cancer cells of the submandibular gland (A 253), Epstein\u2010Barr virus\u2010infected human B cells (BC\u20101) and mouse fibroblasts (3T3) were Incubated with chemotherapeutic drugs for 1 to 4 days at 37\u00b0 C. Despite the microscopic similarities to patient carcinomas, cancer cell lines of the head and neck showed different susceptibilities to cell kill mediated by chemotherapeutic drugs, as compared to in vivo therapeutic results with patients. The nonsquamous carcinoma lines demonstrated high chemosensitive responses after incubation with daunorubicin, cyclophosphamide, dactinomycin, vincristine, and aclarubicin. Surprisingly, only low cell killing rates in squamous carcinoma cell lines were observed after incubation with chemotherapeutic agents such as <jats:italic>cis<\/jats:italic>\u2010platinum, 5\u2010fluorouracil, methotrexate, or bleomycin, which are most commonly used for head and neck cancers. The results show that cytotoxic drug action on in vitro cultured squamous carcinoma cell lines of the head and neck is not representative for the in vivo responses of patient tumors. The cell lines are, however, of potential value for evaluation of cell biochemical changes associated with cytotoxic drug resistance.<\/jats:p>","DOI":"10.1177\/019459988809800603","type":"journal-article","created":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T07:12:06Z","timestamp":1499843526000},"page":"552-557","source":"Crossref","is-referenced-by-count":4,"title":["Multiparametric Flow Cytometry of Human Squamous Cell Carcinoma Lines from the Head and Neck"],"prefix":"10.1002","volume":"98","author":[{"given":"Thomas P.U.","family":"Wustrow","sequence":"first","affiliation":[{"name":"Klinik und Poliklinik fur Hals\u2010Nasen\u2010Ohrenkranke Ludwig\u2010Maximilians\u2010Universitat Klinikum Grosshadern Munchen West Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Raffael","sequence":"additional","affiliation":[{"name":"Arbeitsgruppe Krebszellforschung Max\u2010Planck\u2010Institut fur Biochemie Munchen West Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcnter","family":"Valet","sequence":"additional","affiliation":[{"name":"Arbeitsgruppe Krebszellforschung Max\u2010Planck\u2010Institut fur Biochemie Munchen West Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1988,6]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"128","article-title":"Chemoradio\u2010therapy (CMT) with simultaneous 5\u2010fluorouracil (5FU), cir\u2010platinum (DDP), and radiation (RAD) in the management of squamous cell cancer of the head and neck","volume":"5","author":"Adelstein DJ","year":"1986","journal-title":"Proc Am Soc Clin Oncol"},{"key":"e_1_2_1_3_1","first-page":"933","article-title":"Improved control in advanced head and neck cancer with simultaneous radiation and cisplatin\/5\u2010FU chemotherapy","volume":"69","author":"Taylor SG","year":"1985","journal-title":"Cancer Treat Rep"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-998678"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1986.03780100066010"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016488309130956"},{"key":"e_1_2_1_7_1","first-page":"1657","article-title":"Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas","volume":"41","author":"Rheinwald JG","year":"1981","journal-title":"Cancer Res"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197201)29:1<117::AID-CNCR2820290119>3.0.CO;2-R"},{"key":"e_1_2_1_9_1","first-page":"49","article-title":"Human cytotoxic T lymphocytes. 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Large numbers of peripheral blood T cells clonally developed into allorestricted anti\u2010viral cytotoxic T cell populations in vitro","volume":"3","author":"Kabelitz D","year":"1987","journal-title":"J Mol Cell Immunol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/25.7.330731"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-4097-3_7"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01047331"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0001-8686(80)87003-5"},{"key":"e_1_2_1_14_1","first-page":"125","volume-title":"Flow cytometry IV","author":"Valet G.","year":"1980"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(81)90308-1"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.887927"},{"key":"e_1_2_1_17_1","first-page":"394","article-title":"Genetic and epigenetic mechanisms for the development of metastatic heterogeneity in neoplasms","volume":"26","author":"Kerbel RS","year":"1985","journal-title":"Proc Amer Assoc Cancer Res"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.959840"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00402602"},{"key":"e_1_2_1_20_1","first-page":"45","volume-title":"Electromicroscopical and functional studies of in vitro cultured head and neck cancer cell lines","author":"Wustrow TPU","year":"1984"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-998543"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/019459988809800603","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/019459988809800603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T13:12:01Z","timestamp":1760965921000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1177\/019459988809800603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,6]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1988,6]]}},"alternative-id":["10.1177\/019459988809800603"],"URL":"https:\/\/doi.org\/10.1177\/019459988809800603","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"type":"print","value":"0194-5998"},{"type":"electronic","value":"1097-6817"}],"subject":[],"published":{"date-parts":[[1988,6]]}}}