{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:44:16Z","timestamp":1761007456772,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1990,4,1]],"date-time":"1990-04-01T00:00:00Z","timestamp":638928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE07459"],"award-info":[{"award-number":["DE07459"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[1990,4]]},"abstract":"<jats:p>Isotretinoin (Accutane), a widely used dermatologic drug, produces severe congenital malformations when used during pregnancy. The isotretinoin teratogen syndrome consists of multiple cardiovascular and craniofacial anomalies, most commonly involving the external ear. This study examined the pathogenesis of isotretinoin teratogenicity in a mouse model, using microdissection and histologic examination of fetal mouse ears after treatment with the drug at various stages of embryonic development. In this study, earlier treatment times frequently produced microtia similar to that seen in affected infants, as well as recognizable patterns of temporal bone and ossicular abnormalities; exposure at a later developmental stage resulted in facial tags with less severely affected ears. Possible teratogenic mechanisms of isotretinoin are discussed. Suitability of the mouse model for studying human congenital craniofacial malformations, such as Goldenhar's and Treacher Collins Syndrome, is also explored.<\/jats:p>","DOI":"10.1177\/019459989010200414","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T06:42:21Z","timestamp":1448433741000},"page":"391-401","source":"Crossref","is-referenced-by-count":39,"title":["Congenital Malformations of the External Middle, and Inner Ear Produced by Isotretinoin Exposure in Mouse Embryos"],"prefix":"10.1002","volume":"102","author":[{"given":"Bennie L.","family":"Jarvis","sequence":"first","affiliation":[{"name":"Department of Otolaryngology West Virginia University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.C.","family":"Johnston","sequence":"additional","affiliation":[{"name":"Department of Anatomy West Virginia University"},{"name":"Department of Ophthalmology West Virginia University"},{"name":"Department of Orthodontics University of North Carolina"},{"name":"Department of Dental Research Center University of North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.K.","family":"Sulik","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology West Virginia University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1990,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1984.03340480049027"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198510033131401"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.117.3046.535"},{"key":"e_1_2_1_5_1","first-page":"211","article-title":"Isotretinoin embryopathy and the cranial neural crest: An in vivo and in vitro study","volume":"6","author":"Webster WS","year":"1986","journal-title":"J Craniofac Genet Dev 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