{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:20:39Z","timestamp":1761060039557,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1991,3,1]],"date-time":"1991-03-01T00:00:00Z","timestamp":667785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[1991,3]]},"abstract":"<jats:p>A human squamous cell carcinoma (SCC) from the floor of the mouth (FOM) was implanted by a needle aspiration technique in the FOM site of athymic nude mice. Mice were killed at 3\u2010week intervals, and the oral cavity, mandible, and neck were sectioned and examined histologically. Tumor growth was observed in 65% of the animals, with histologic features consistent with the engrafted human invasive SCC. These features included invasion of connective tissue in 92%, invasion of muscle in 77%, invasion and destruction of bone in 54%, and vascular invasion in 15% of the mice. In contrast, FOM tumor implanted subcutaneously on back sites of nude mice was totally encapsulated by fibrous connective tissue with evidence of capsular invasion. SCC from other head and neck sites showed similar locally invasive growth after intraoral implantation in nude mice. The results demonstrate the invasive characteristics of human head and neck SCC grown in the homologous oral cavity site in nude mice and support the nude mouse as a biologically relevant in vivo model in the investigation of the biologic characteristics and therapy of head and neck carcinoma.<\/jats:p>","DOI":"10.1177\/019459989110400310","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T06:43:17Z","timestamp":1448433797000},"page":"351-357","source":"Crossref","is-referenced-by-count":6,"title":["Growth and histopathology of human head and neck squamous cell carcinoma implanted intraorally in nude mice"],"prefix":"10.1002","volume":"104","author":[{"given":"Elizabeth W.","family":"Sales","sequence":"first","affiliation":[{"name":"Head and Neck Tumor Biology Program Departments of Pathology Eastern Virginia Medical School Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen A.","family":"Fitch","sequence":"additional","affiliation":[{"name":"Otolaryngology\u2010Head and Neck Surgery Eastern Virginia Medical School Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela A.","family":"Sudetic","sequence":"additional","affiliation":[{"name":"Otolaryngology\u2010Head and Neck Surgery Eastern Virginia Medical School Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary L.","family":"Schechter","sequence":"additional","affiliation":[{"name":"Otolaryngology\u2010Head and Neck Surgery Eastern Virginia Medical School Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Faulconer","sequence":"additional","affiliation":[{"name":"Head and Neck Tumor Biology Program Departments of Pathology Eastern Virginia Medical School Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth D.","family":"Somers","sequence":"additional","affiliation":[{"name":"Otolaryngology\u2010Head and Neck Surgery Eastern Virginia Medical School Norfolk Virginia"},{"name":"Microbiology\/Immunology Eastern Virginia Medical School Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1991,3]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"2","article-title":"Epidemiology of head and neck cancer","volume":"15","author":"Blitzer PH","year":"1988","journal-title":"Semin Oncol"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/80.9.688"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00454264"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016488309130933"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0277-5379(86)90323-8"},{"key":"e_1_2_1_7_1","first-page":"187","volume-title":"Head and neck oncology research","author":"Fitch KA","year":"1988"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1973.41"},{"key":"e_1_2_1_9_1","first-page":"3186","article-title":"Growth patterns and metastatic behavior of human tumor growing in athymic mice","volume":"38","author":"Kryiazis AP","year":"1978","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_1","first-page":"367","article-title":"Collagenase inhibitors retarding invasion of a human tumor in nude mice","volume":"48","author":"De Vore DP","year":"1980","journal-title":"Exp Cell Biol"},{"key":"e_1_2_1_11_1","first-page":"377","article-title":"Different growth pattern and biologic behavior of human renal cell carcinoma implanted into different organs of nude mice","volume":"78","author":"Naito S","year":"1987","journal-title":"J Natl Cancer Inst"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00058059"},{"key":"e_1_2_1_13_1","first-page":"1928","article-title":"Metastatic behavior of tumor cells isolated from primary and metastatic human colorectal carcinomas implanted into different sites in nude mice","volume":"46","author":"Giavazzi R","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_1_14_1","first-page":"209","article-title":"Metastases of human tumor xenografts in nude mice","volume":"4","author":"Neulat\u2010Duga I","year":"1984","journal-title":"Invasion Metastasis"},{"key":"e_1_2_1_15_1","first-page":"238","article-title":"Influence of implantation site on growth, antigen expression and metastatic potential of human colonic cancer HT29 and 5583 xenografts in nude mice","volume":"8","author":"Sekikawa K","year":"1988","journal-title":"Invasion Metastasis"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/56.6.1251"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/019459989110400310","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/019459989110400310","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T13:21:52Z","timestamp":1760966512000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1177\/019459989110400310"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,3]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,3]]}},"alternative-id":["10.1177\/019459989110400310"],"URL":"https:\/\/doi.org\/10.1177\/019459989110400310","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"type":"print","value":"0194-5998"},{"type":"electronic","value":"1097-6817"}],"subject":[],"published":{"date-parts":[[1991,3]]}}}