{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:34:54Z","timestamp":1761006894033,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1992,10,1]],"date-time":"1992-10-01T00:00:00Z","timestamp":717897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P50\u2010DC00133","T32\u2010DC00009\u201007084"],"award-info":[{"award-number":["P50\u2010DC00133","T32\u2010DC00009\u201007084"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:p>Previous experiments have shown that Pseudomonas aeruginosa may infect the middle ears of chinchillas by way of the eustachian tube and that chinchillas with acute otitis media (AOM) are more susceptible to pseudomonas infection than animals without AOM. The purpose of this experiment was to examine the effects of otitis media with effusion (OME), induced by means of eustachian tube obstruction, on middle ear susceptibility to nasal inoculation of P. aeruginosa. Chinchilla eustachian tubes were obstructed with silicone rubber sponge bilaterally; OME developed in eight animals (11 ears)\u2014three bilaterally and five unilaterally\u2014and persisted for 6 months. Ten chinchillas with normal eustachian tube function served as controls. All animals were nasally inoculated with 5 times 10<jats:sup>4<\/jats:sup> colony\u2010forming units of P. aeruginosa. Pseudomonas otitis media developed in eight of 11 OME ears with effusion, none of five ears without OME, and four of 20 control ears (X<jats:sup>2<\/jats:sup> = 11.782, <jats:italic>p<\/jats:italic> = 0.003). Therefore, P. aeruginosa can infect the middle ear by way of the eustachian tube. Tubal dysfunction may lead to the development of chronic suppurative otitis media by increasing tubotympanic susceptibility to opportunistic pathogens.<\/jats:p>","DOI":"10.1177\/019459989210700402","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T06:46:48Z","timestamp":1448434008000},"page":"511-515","source":"Crossref","is-referenced-by-count":10,"title":["Pseudomonas Otitis Media after Eustachian Tube Obstruction"],"prefix":"10.1002","volume":"107","author":[{"given":"Patrick J.","family":"Antonelli","sequence":"first","affiliation":[{"name":"Otitis Media Research Center Department of Otolaryngology University of Minnesota School of Medicine Minneapolis Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven K.","family":"Juhn","sequence":"additional","affiliation":[{"name":"Otitis Media Research Center Department of Otolaryngology University of Minnesota School of Medicine Minneapolis Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos V.","family":"Goycoolea","sequence":"additional","affiliation":[{"name":"Otitis Media Research Center Department of Otolaryngology University of Minnesota School of Medicine Minneapolis Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Scott","family":"Giebink","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Minnesota School of Medicine Minneapolis Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,10]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"487","volume-title":"Pediatric otolaryngology","author":"Bluestone CD","year":"1990"},{"key":"e_1_2_1_3_1","first-page":"213","volume-title":"Models in the evaluation of chemotherapy of infectious diseases","author":"Giebink GS","year":"1986"},{"key":"e_1_2_1_4_1","article-title":"Middle ear susceptibility to pseudomonas infection during acute otitis media","author":"Antonelli PJ","year":"1992","journal-title":"Presented at the Association for Research in Otolaryngology 14th Midwinter Meeting, St. Petersburg Beach, Fla. February 1991. 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Presented at the Association for Research in Otolaryngology 15th Midwinter Meeting St. Petersburg Beach Fla February1992."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1985.00800070057007"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348947007900409"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348947708600407"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016488409122881"},{"key":"e_1_2_1_12_1","unstructured":"PizaM SchachernPA SanoS PaparellaMM.Eustachian tube obstructions: A longitudinal study in cats and chinchillas. In: Lim D ed. Abstracts of the Fifth International Symposium Recent Advances in Otitis Media Ft. 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