{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T08:11:31Z","timestamp":1778487091092,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Tuberculosis and Lung Diseases Research Center of Tabriz University of Medical Sciences, Tabriz, Iran","award":["62565"],"award-info":[{"award-number":["62565"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Nutr Health"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Chronic high-fat diet (HFD) consumption is linked to chronic kidney disease. Toll-like receptor 4 (TLR4) promotes pro-fibrotic and pro-inflammatory pathways in the kidney. It is directly inhibited by renal miR-874.\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -Chalcone has protective effects against HFD-related diseases. However, its effects on renal miR-874 and TLR4 expression in HFD-fed animals remain unexamined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      The current study examined the possible influences of\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone and HFD on the renal miR-874\/TLR4 axis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Male rats were divided into control, HFD, and HFD\u2009+\u2009\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone groups. They received Tween 80 in control, high-fat emulsion in HFD and HFD\u2009+\u2009\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone groups, and\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone in the HFD\u2009+\u2009\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone group. Fasting insulin resistance index (FIRI) was calculated, and real-time PCR was used to determine the expression levels of miR-874 and TLR4 in the kidneys.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Chronic HFD feeding elevated FIRI levels. In contrast, co-treatment with\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone and HFD significantly reduced FIRI levels in the HFD\u2009+\u2009\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone group compared with the HFD group. Consumption of an HFD also significantly reduced miR-874 and increased TLR4 expression. These HFD-induced changes were inhibited by\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone. There were no significant differences in renal expression of miR-874 and TLR4 between the control and HFD\u2009+\u2009\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Down-regulation of renal miR-874 may be responsible for elevated TLR4 mRNA levels during HFD feeding. Furthermore, miR-874 dysregulation may be linked to the development of HFD-related kidney disease. This study suggests that\n                      <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                      -chalcone is a potential therapeutic chalcone for protecting the kidney against the dysregulation of the miR-874\/TLR4 pathway during HFD feeding.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/02601060261446821","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T14:35:56Z","timestamp":1778250956000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>trans<\/i>\n                    -Chalcone protects against high-fat diet-induced kidney injury in rats by regulating the miR-874\/TLR4 pathway"],"prefix":"10.1177","author":[{"given":"Elham","family":"Karimi-Sales","sequence":"first","affiliation":[{"name":"Tabriz University of Medical Sciences"},{"name":"Tabriz University of Medical Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalil","family":"Hajiasgharzadeh","sequence":"additional","affiliation":[{"name":"Tabriz University of Medical Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6484-4267","authenticated-orcid":false,"given":"Mohammad Reza","family":"Alipour","sequence":"additional","affiliation":[{"name":"Tabriz University of Medical Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-022-07174-x"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v13.i3.129"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222111306"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)92143-5"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31694-5"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000534842"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000484386"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S326975"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.4c00368"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.196"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO00923F"},{"issue":"1","key":"e_1_3_3_13_1","first-page":"212563","article-title":"TLR4 and insulin resistance","volume":"2010","author":"Kim JJ","year":"2010","unstructured":"Kim JJ, Sears DD (2010) TLR4 and insulin resistance. 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