{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:59:06Z","timestamp":1777449546693,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2004,6,1]],"date-time":"2004-06-01T00:00:00Z","timestamp":1086048000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Altern Lab Anim"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>\n                    Following on from previous studies on dermal inflammation in the isolated perfused bovine udder, a new in vitro model of the isolated haemoperfused bovine uterus was established for studies on acute inflammatory reactions (for example, eicosanoid synthesis and regulation of cyclooxygenase-1 [COX-1] and COX-2) caused by ischaemia\u2013reperfusion (I\u2013R) injury. The organs and blood used in this study were obtained from a slaughterhouse. Within 2 hours of slaughter, uterine perfusion was re-established, by using a mixture of homologous blood and Tyrode solution (4:1). After equilibration, several deposits of arachidonic acid (5mg and 0.1mg) and arachidonylethanolamide (0.1mg) were injected into the myometrial tissue. Tissue biopsies were taken from treated and untreated areas at 180 and 300 minutes after the onset of haemoperfusion, for measuring prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    (PGE\n                    <jats:sub>2<\/jats:sub>\n                    ) levels. In addition, the regulation of COX-1 and COX-2 mRNA was investigated by using the reverse transcriptase-polymerase chain reaction. Eicosanoid levels were determined by using an enzyme immunoassay (ELISA). Because both an increase in PGE\n                    <jats:sub>2<\/jats:sub>\n                    concentration and up-regulation of COX mRNA were observed, the inhibitory effects of dexamethasone, added to the perfusion medium, were studied. Dexamethasone caused a significant decrease in tissue PGE\n                    <jats:sub>2<\/jats:sub>\n                    production, but did not induce down-regulation of COX-2 mRNA. In conclusion, the isolated haemoperfused bovine uterus was introduced as an in vitro model of acute inflammation, induced by I\u2013R injury. The suitability of the model for investigating anti-inflammatory substances was demonstrated. Use of the isolated haemoperfused bovine uterus in pharmacological research and drug screening may contribute to reducing the number of animals used for testing.\n                  <\/jats:p>","DOI":"10.1177\/026119290403200204","type":"journal-article","created":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:27:11Z","timestamp":1554856031000},"page":"69-77","source":"Crossref","is-referenced-by-count":4,"title":["Ischaemia\u2013Reperfusion Injury in the Isolated Haemoperfused Bovine Uterus: An\n                    <i>In Vitro<\/i>\n                    Model of Acute Inflammation"],"prefix":"10.1177","volume":"32","author":[{"given":"Michael","family":"Braun","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Toxicology and Pharmacy, School of Veterinary Medicine, Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Kietzmann","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Toxicology and Pharmacy, School of Veterinary Medicine, Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2004,6,1]]},"reference":[{"key":"bibr1-026119290403200204","first-page":"57","volume":"19","author":"B\u00e4umer W.","year":"2002","journal-title":"Alternativen zu Tierexperimenten"},{"key":"bibr2-026119290403200204","volume-title":"Untersuchungen zur Schleimhautvertr\u00e4glichkeit von Testsubstanzen am Modell des isoliert perfundierten Rinderuterus.","author":"Mertens A.","year":"2001"},{"key":"bibr3-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1211\/0022357011775875"},{"key":"bibr4-026119290403200204","first-page":"643","volume":"28","author":"B\u00e4umer W.","year":"2000","journal-title":"ATLA"},{"key":"bibr5-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99433-0"},{"key":"bibr6-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.300.2.367"},{"key":"bibr7-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44821-6"},{"key":"bibr8-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114850"},{"key":"bibr9-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.7.2692"},{"key":"bibr10-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(95)00194-8"},{"key":"bibr11-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200002)190:3<255::AID-PATH526>3.0.CO;2-6"},{"key":"bibr12-026119290403200204","doi-asserted-by":"publisher","DOI":"10.2174\/1568014024606511"},{"key":"bibr13-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2760(97)00129-X"},{"key":"bibr14-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.34.21181"},{"key":"bibr15-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105854200"},{"key":"bibr16-026119290403200204","first-page":"1172","volume-title":"Methoden der Enzymatischen Analyse","author":"Bergmeyer H.U.","year":"1970"},{"key":"bibr17-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1039\/an9729700142"},{"key":"bibr18-026119290403200204","volume-title":"Der isoliert h\u00e4mopefundierte Rinderuterus als In-vitro-Modell zur Pr\u00fcfung anti-inflammatorischer Substanzen.","author":"Braun M.","year":"2002"},{"key":"bibr19-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"bibr20-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/1056-8719(93)90010-C"},{"key":"bibr21-026119290403200204","volume-title":"Isoliert perfundiertes Rindereuter \u2014 Ein Modell zur Pr\u00fcfung der Hautvertr\u00e4glichkeit?","author":"Maass P.","year":"1993"},{"key":"bibr22-026119290403200204","volume-title":"Untersuchung haut- und schleimhautirritierender Eigenschaften von Testsubstanzen am isoliert perfundierten Rindereuter.","author":"Sterl F.","year":"1998"},{"key":"bibr23-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.277.3.H1207"},{"key":"bibr24-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(96)00694-7"},{"key":"bibr25-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1997.0572"},{"key":"bibr26-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702966"},{"key":"bibr27-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)90599-1"},{"key":"bibr28-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(95)00168-9"},{"key":"bibr29-026119290403200204","first-page":"1069","volume":"283","author":"Zhang Y.","year":"1997","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"bibr30-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00377-0"},{"key":"bibr31-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1007\/PL00007899"},{"key":"bibr32-026119290403200204","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(99)00681-0"},{"key":"bibr33-026119290403200204","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.1.469"}],"container-title":["Alternatives to Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/026119290403200204","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/026119290403200204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:41:18Z","timestamp":1777380078000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/026119290403200204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["10.1177\/026119290403200204"],"URL":"https:\/\/doi.org\/10.1177\/026119290403200204","relation":{},"ISSN":["0261-1929","2632-3559"],"issn-type":[{"value":"0261-1929","type":"print"},{"value":"2632-3559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6]]}}}