{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:47:44Z","timestamp":1777448864437,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[1999,5,1]],"date-time":"1999-05-01T00:00:00Z","timestamp":925516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Altern Lab Anim"],"published-print":{"date-parts":[[1999,5]]},"abstract":"<jats:p>\n                    Environmental mercury and mercury compound contamination has increased dramatically since the industrial revolution. This paper describes the toxic effects of mercury on a culture of hybridoma TA7 cells, which produce antibodies against the A-subunit of viskumin. Cells were cultivated on 96-well flat-bottomed plates with RPMI-1640 medium supplemented with 10% fetal calf serum at 37\u00b0C in 5% CO\n                    <jats:sub>2<\/jats:sub>\n                    \/95% air. The cells were exposed to 0.1nM\/l\u201310\u03bcM\/l Hg\n                    <jats:sub>2<\/jats:sub>\n                    (NO\n                    <jats:sub>3<\/jats:sub>\n                    )\n                    <jats:sub>2<\/jats:sub>\n                    .2H\n                    <jats:sub>2<\/jats:sub>\n                    O (mercury nitrate) during the exponential growth phase. Toxicity was assessed by using the colorimetric MTT (tetrazolium) assay after exposure for 48 hours. Cell growth and cell survival were evaluated by using percentage indices of cellular content in exposed cells when compared to non-exposed control cells. The concentrations of the no-effect level, the lowest observed effect level and the the highest toxic effect level were registered. The toxic effects of the mercury compound on the hybridoma cells occurred between 0.1\u03bcM\/l and 10\u03bcM\/l.\n                  <\/jats:p>","DOI":"10.1177\/026119299902700315","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T05:15:25Z","timestamp":1554786925000},"page":"397-401","source":"Crossref","is-referenced-by-count":0,"title":["Toxicity of Mercury to Hybridoma TA7 Cells"],"prefix":"10.1177","volume":"27","author":[{"given":"Inessa","family":"Remez","sequence":"first","affiliation":[{"name":"Institute of Occupational and Environmental Health, Medical Academy of Latvia, 16 Dzierciema Street, Riga 1007, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pauls","family":"Andersons","sequence":"additional","affiliation":[{"name":"Latvian University of Agriculture, 2 Liela Street, Jelgava 3001, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hackel","family":"Veksler","sequence":"additional","affiliation":[{"name":"Institute of Occupational and Environmental Health, Medical Academy of Latvia, 16 Dzierciema Street, Riga 1007, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1999,5,1]]},"reference":[{"key":"bibr1-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/S0048-9697(98)00175-2"},{"key":"bibr2-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jhh.1000589"},{"key":"bibr3-026119299902700315","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1001\/jama.1983.03330330080048","volume":"249","author":"Kulig K.","year":"1983","journal-title":"Journal of the American Medical Association"},{"key":"bibr4-026119299902700315","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-76-1-119"},{"key":"bibr5-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1126\/science.172.3987.1027"},{"key":"bibr6-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/S0048-9697(98)00062-X"},{"key":"bibr7-026119299902700315","first-page":"28","volume":"77","author":"Hughes J.","year":"1995","journal-title":"Journal of the Michigan Dental Association"},{"key":"bibr8-026119299902700315","first-page":"40","volume":"64","author":"Kostiniak P.J.","year":"1998","journal-title":"New York State Dental Journal"},{"key":"bibr9-026119299902700315","unstructured":"Medical Statistical Bureau of Latvia (1999). Annual Book of Medical Statistical Bureau of Latvia 42, 46\u201356 (Latvian)."},{"key":"bibr10-026119299902700315","first-page":"112","volume":"54","author":"Salonen J.T.","year":"1998","journal-title":"Bibliotheca Nutritio et Dieta"},{"key":"bibr11-026119299902700315","first-page":"233","volume":"36","author":"Deleu D.","year":"1998","journal-title":"Peripheral polineuropathy due to chronic use of topical ammoniated mercury."},{"issue":"2","key":"bibr12-026119299902700315","first-page":"617","volume":"26","author":"Ekwall B.","year":"1998","journal-title":"ATLA"},{"issue":"1","key":"bibr13-026119299902700315","first-page":"251","author":"Clemedson C.","year":"1996","journal-title":"ATLA"},{"key":"bibr14-026119299902700315","unstructured":"HaitovR.M., SidorovichI.G. & IgnatyevaG.A. (1987). The contemporary aspects of hybridoma's technology. Medicine and Public Health Series: The reviews about important problems of medicine. Moscow, BHUUMU, Part 4, 65 (Russian)."},{"key":"bibr15-026119299902700315","first-page":"20","volume":"5","author":"Maluchenko N.B.","year":"1999","journal-title":"Journal of Molecular Biology"},{"key":"bibr16-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"bibr17-026119299902700315","unstructured":"RemezI., VasilyevaS. & AndersonsP. (1995). Some modern methods in ecotoxicology.\n                      Eighth Scandinavian Workshop on In Vitro Toxicology 1995.\n                      Tampere, Finland. Abstract 131."},{"key":"bibr18-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(98)00058-4"},{"key":"bibr19-026119299902700315","first-page":"86","volume":"43","author":"Purchase I.F.H.","year":"1998","journal-title":"Toxicological Science"},{"key":"bibr20-026119299902700315","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4049\/jimmunol.150.1.8","volume":"150","author":"Setum C.","year":"1993","journal-title":"Journal of Immunology"},{"key":"bibr21-026119299902700315","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4049\/jimmunol.150.1.8","volume":"150","author":"Gomi H.","year":"1993","journal-title":"Journal of Immunology"},{"key":"bibr22-026119299902700315","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4049\/jimmunol.150.1.8","volume":"150","author":"Mori A.","year":"1993","journal-title":"Journal of Immunology"},{"key":"bibr23-026119299902700315","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4049\/jimmunol.150.1.8","volume":"150","author":"Suzuki R.","year":"1993","journal-title":"Journal of Immunology"},{"key":"bibr24-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-2333(98)00004-6"},{"key":"bibr25-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-2333(98)80006-4"},{"key":"bibr26-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-2333(97)00115-X"},{"key":"bibr27-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1998.8407"},{"key":"bibr28-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(92)90224-H"},{"key":"bibr29-026119299902700315","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-2333(97)00107-0"}],"container-title":["Alternatives to Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/026119299902700315","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/026119299902700315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:39:12Z","timestamp":1777379952000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/026119299902700315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,5]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,5]]}},"alternative-id":["10.1177\/026119299902700315"],"URL":"https:\/\/doi.org\/10.1177\/026119299902700315","relation":{},"ISSN":["0261-1929","2632-3559"],"issn-type":[{"value":"0261-1929","type":"print"},{"value":"2632-3559","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,5]]}}}