{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T17:27:16Z","timestamp":1777915636844,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2016,7,9]],"date-time":"2016-07-09T00:00:00Z","timestamp":1468022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Perfusion"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Several factors have been historically advocated to explain the coagulative and inflammatory disorders following cardiopulmonary bypass (CPB). In this paper, we describe the presence of circulating non-hematological cells, introduced within the bloodstream during CPB. We defined the origin of the cells and tested their impact on coagulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>We collected peripheral arterial blood samples in twenty consecutive coronary artery bypass graft cases at four different surgical moments and assessed the presence and nature of circulating cells with the use of the CELLSEARCH\u00ae Test, immunocytochemistry and immunofluorescence, evaluating the expression of cytokeratin and calretinin. The effect of the circulating non-hematological cells on coagulation was tested in vitro, using the ROTEM assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>A mean of 263.85 \u00b1 57.5 (median 258.5) cells were present in the samples following the suction of blood from the surgical field while all the other samples were negative (zero cells) (p&lt;0.00001). Immunologic tests confirmed the mesothelial origin of the cells. The ROTEM\u00ae assay of the blood samples contaminated by the mesothelial cells presented longer clotting times (53.4 \u00b1 8.2 secs 48.3 \u00b1 8.9 sec, p=0.05), longer clot formation times (137.1 \u00b1 31.5 sec vs 111.9 \u00b1 25.2 sec, p=0.009), smaller alfa angle amplitudes (66.7 \u00b1 9.1\u00b0 vs 71.1 \u00b1 5.1\u00b0, p=0.04) and maximum clot firmness times (59.0 \u00b1 5.4 sec vs 61.9 \u00b14.6 sec, p=0.004) than the controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The presence of circulating non-hematological cells during CPB with a mesothelial immunophenotype alters in vitro coagulation assays. This finding can help to further understand the pathophysiology of CPB.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0267659116638916","type":"journal-article","created":{"date-parts":[[2016,3,21]],"date-time":"2016-03-21T21:18:25Z","timestamp":1458595105000},"page":"584-592","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Circulating non-hematological cells during cardiopulmonary bypass: new findings in cardiac surgery procedures"],"prefix":"10.1177","volume":"31","author":[{"given":"Gianluca","family":"Santise","sequence":"first","affiliation":[{"name":"Cardiothoracic Surgery Unit, Sant\u2019Anna Hospital, Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cinzia","family":"Marinaro","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Catanzaro, \u201cMagna Graecia\u201d, Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele","family":"Maselli","sequence":"additional","affiliation":[{"name":"Cardiothoracic Surgery Unit, Sant\u2019Anna Hospital, Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmelo","family":"Dominici","sequence":"additional","affiliation":[{"name":"Cardiothoracic Surgery Unit, Sant\u2019Anna Hospital, Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Di Vito","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Catanzaro, \u201cMagna Graecia\u201d, Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Donato","sequence":"additional","affiliation":[{"name":"Department of Health Science, Pathology Unit, University of Catanzaro, \u201cMagna Graecia\u201d, Catanzaro, 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