{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T17:31:24Z","timestamp":1777915884520,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2022,9,3]],"date-time":"2022-09-03T00:00:00Z","timestamp":1662163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2021206031"],"award-info":[{"award-number":["H2021206031"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]},{"name":"S&T Program of Hebei","award":["203777117D and 19277757D"],"award-info":[{"award-number":["203777117D and 19277757D"]}]},{"name":"Key Science and Technology Research Program of Hebei Provincial Health Commission","award":["20210065"],"award-info":[{"award-number":["20210065"]}]},{"name":"Hebei provincial government funded specialty capacity building and specialty leader training Project","award":["LS201808"],"award-info":[{"award-number":["LS201808"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Perfusion"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>To explore impact of various periods of ischemia and reperfusion on the severity of myocardial injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Langendorff model of isolated cardiac perfusion system was established in 56 rat hearts. They were randomly assigned into four groups with four different ischemia (perfusion-pause) time and reperfusion time. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and creatine kinase-MB (CK-MB) were measured and the size of myocardial infarction was assessed by 2,3,5-triphenyltetrazolium chloride (TTC) staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The levels of AST, ALT, LDH, and CK-MB in the heart tissues and perfusate were lowest in the group I (shortest time of perfusion-pause and reperfusion) followed by the groups II, III, and IV (longest time of perfusion-pause and reperfusion) ( p &lt; 0.05). The myocardial infarction size was smallest in the group I (6.63 \u00b1 0.47) followed by group II (15.12 \u00b1 1.03), group III (20.32 \u00b1 2.18), and group IV (32.29 \u00b1 5.42) ( p &lt; 0.05). Two-way ANOVA analysis revealed that period of perfusion-pause and reperfusion independently and significantly affected the levels of AST and ALT in both heart tissues and perfusate ( p &lt; 0.001). The interaction of pausing period and reperfusion significantly affected the level of AST ( p = 0.046) and CK-MB ( p = 0.001) in the perfusate. In addition, perfusion-pause period significantly affected levels of LDH and CK-MB only in the perfusate ( p &lt; 0.001). Neither perfusate nor heart tissue LDH level was significantly affected by the interaction of perfusion-pause and reperfusion period ( p &gt; 0.05).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The severity of myocardial injury in the Langendorff model was affected by the period of perfusion-pause and reperfusion. The longer period of perfusion-pause followed by the longer the period of reperfusion, the severe myocardial injury was found.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/02676591221122349","type":"journal-article","created":{"date-parts":[[2022,9,3]],"date-time":"2022-09-03T03:19:23Z","timestamp":1662175163000},"page":"1609-1616","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Impact of various periods of perfusion-pause and reperfusion on the severity of myocardial injury in the langenodorff model"],"prefix":"10.1177","volume":"38","author":[{"given":"Le","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiology, The First Hospital of Hebei Medical University, Shijiazhuang, China"},{"name":"Hebei Key Laboratory of Heart and Metabolism, Shijiazhuang, Hebei, 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