{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T01:30:13Z","timestamp":1777858213162,"version":"3.51.4"},"reference-count":71,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T00:00:00Z","timestamp":1559606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"L\u2019Oreal Italia"},{"name":"Italian Ministry of University and Research","award":["PRIN-2012JTX3KL, PRIN2010-11 n. 20103W4779_003"],"award-info":[{"award-number":["PRIN-2012JTX3KL, PRIN2010-11 n. 20103W4779_003"]}]},{"DOI":"10.13039\/501100010739","name":"universit\u00e1 degli studi di camerino","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Psychopharmacol"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Pharmacological treatment approaches for eating disorders, such as binge eating disorder and bulimia nervosa, are currently limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and aims:<\/jats:title>\n                    <jats:p>\n                      Using a well-characterized animal model of binge eating, we investigated the epigenetic regulation of the A\n                      <jats:sub>2A<\/jats:sub>\n                      Adenosine Receptor (A\n                      <jats:sub>2A<\/jats:sub>\n                      AR) and dopaminergic D2 receptor (D2R) genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Gene expression analysis revealed a selective increase of both receptor mRNAs in the amygdaloid complex of stressed and restricted rats, which exhibited binge-like eating, when compared to non-stressed and non-restricted rats. Consistently, pyrosequencing analysis revealed a significant reduction of the percentage of DNA methylation but only at the A\n                      <jats:sub>2A<\/jats:sub>\n                      AR promoter region in rats showing binge-like behaviour compared to the control animals. Focusing thus on A\n                      <jats:sub>2A<\/jats:sub>\n                      AR agonist (VT 7) administration (which inhibited the episode of binge systemically at 0.1 mg\/kg or intra-central amygdala (CeA) injection at 900 ng\/side) induced a significant increase of A\n                      <jats:sub>2A<\/jats:sub>\n                      AR mRNA levels in restricted and stressed rats when compared to the control group. In addition, we observed a significant decrease in A\n                      <jats:sub>2A<\/jats:sub>\n                      AR mRNA levels in rats treated with the A\n                      <jats:sub>2A<\/jats:sub>\n                      AR antagonist (ANR 94) at 1 mg\/kg. Consistent changes in the DNA methylation status of the A\n                      <jats:sub>2A<\/jats:sub>\n                      AR promoter were found in restricted and stressed rats after administration of VT 7 or ANR 94.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      We confirm the role of A\n                      <jats:sub>2A<\/jats:sub>\n                      AR in binge eating behaviours, and we underline the importance of epigenetic regulation of the A\n                      <jats:sub>2A<\/jats:sub>\n                      AR gene, possibly due to a compensatory mechanism to counteract the effect of binge eating. We suggest that A\n                      <jats:sub>2A<\/jats:sub>\n                      AR activation, inducing receptor gene up-regulation, could be relevant to reduction of food consumption.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0269881119845798","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T09:19:51Z","timestamp":1559639991000},"page":"1550-1561","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":26,"title":["Regulation of adenosine A\n                    <sub>2A<\/sub>\n                    receptor gene expression in a model of binge eating in the amygdaloid complex of female rats"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8044-1206","authenticated-orcid":false,"given":"Maria Vittoria","family":"Micioni Di Bonaventura","sequence":"first","affiliation":[{"name":"School of Pharmacy, Pharmacology Unit, University of Camerino, Camerino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariangela","family":"Pucci","sequence":"additional","affiliation":[{"name":"Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Elena","family":"Giusepponi","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Pharmacology Unit, University of Camerino, Camerino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adele","family":"Romano","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology V. Erspamer, Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catia","family":"Lambertucci","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Camerino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosaria","family":"Volpini","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Camerino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuela","family":"Micioni Di Bonaventura","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Pharmacology Unit, University of Camerino, Camerino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Gaetani","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology V. 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