{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T01:50:24Z","timestamp":1788918624376,"version":"build-2803163510"},"reference-count":21,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T00:00:00Z","timestamp":1619049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Psychopharmacol"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Dopamine transporter (DAT) and serotonin transporter (SERT) are targets for many psychoactive substances. Functional assays including uptake inhibition and release assays often involve radiolabeled compounds like [\n                      <jats:sup>3<\/jats:sup>\n                      H]-dopamine and [\n                      <jats:sup>3<\/jats:sup>\n                      H]-serotonin to assess drug activity at transporters, which have high requirements on handling radioactive samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>The aim of this study was to establish a label-free method to assess drug activity at DAT and SERT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A liquid chromatography coupled with tandem mass spectrometry (LC-MS\/MS) method was established using transporter-transfected human embryonic kidney 293T (HEK293T) cells. This method was evaluated by testing the effects of amphetamine and cocaine in the assay procedure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The limits of detection of this method were 0.2\u2009nM for both dopamine (DA) and serotonin (5-HT), with good linearities in the range of 0.5\u2013160\u2009nM. Amphetamine and cocaine\u2019s IC\n                      <jats:sub>50<\/jats:sub>\n                      and EC\n                      <jats:sub>50<\/jats:sub>\n                      on DAT and SERT determined by this method were consistent with previous reports.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>A rapid, reliable and label-free LC-MS\/MS method for assessing drug activity was established, which affords an attractive alternative for those laboratories that do not have a radiation license or capabilities.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/02698811211008576","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T01:58:53Z","timestamp":1619143133000},"page":"693-700","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["An efficient and label-free LC-MS\/MS method for assessing drug\u2019s activity at dopamine and serotonin transporters using transporter-transfected HEK293T cells"],"prefix":"10.1177","volume":"35","author":[{"given":"Zebin","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, China"},{"name":"Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Center for Quantitative Biology (CQB), Peking University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0626-8425","authenticated-orcid":false,"given":"Chenyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, China"},{"name":"Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Center for Quantitative Biology (CQB), Peking University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enshan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Sciences, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuiqing","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Sciences, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5728-7888","authenticated-orcid":false,"given":"Yulan","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,4,22]]},"reference":[{"key":"bibr1-02698811211008576","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2011.304"},{"key":"bibr2-02698811211008576","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2018.09.006"},{"key":"bibr3-02698811211008576","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2012.204"},{"key":"bibr4-02698811211008576","unstructured":"Committee for Medicinal Products for Human Use (CHMP) (2011) Guideline on Bioanalytical Method Validation. 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