{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T01:31:55Z","timestamp":1777858315392,"version":"3.51.4"},"reference-count":69,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["DA005274"],"award-info":[{"award-number":["DA005274"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["DA007027"],"award-info":[{"award-number":["DA007027"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["DA032246"],"award-info":[{"award-number":["DA032246"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["GM57481"],"award-info":[{"award-number":["GM57481"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["R25GM090084"],"award-info":[{"award-number":["R25GM090084"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Psychopharmacol"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Because of their implications in several pathological conditions, \u03b14\u03b22* nicotinic acetylcholine receptors (nAChRs) are potential targets for the treatment of nicotine dependence, pain, and many psychiatric and neurodegenerative diseases. However, they exist in various subtypes, and finding selective tools to investigate them has proved challenging. The nicotinic receptor agonist, 5-iodo-A-85380 (5IA), has helped in delineating the function of \u03b22-containing subtypes in vitro; however, much is still unknown about its behavioral effects. Furthermore, its effectiveness on \u03b16-containing subtypes is limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>To investigate the effects of 5IA on nociception (formalin, hot-plate, and tail-flick tests), locomotion, hypothermia, and conditioned reward after acute and repeated administration, and to examine the potential role of \u03b22 and \u03b16 nAChR subunits in these effects. Lastly, its selectivity for expressed low sensitivity (LS) and high sensitivity (HS) \u03b14\u03b22 receptors is investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>5IA dose-dependently induced hypothermia, locomotion suppression, conditioned place preference, and antinociception (only in the formalin test but not in the hot-plate or tail-flick tests). Furthermore, these effects were mediated by \u03b22 but not \u03b16 nicotinic subunits. Finally, we show that 5-iodo-A-85380 potently activates both stoichiometries of \u03b14\u03b22 nAChRs with differential efficacies, being a full agonist on HS \u03b14(2)\u03b22(3) nAChRs, and a partial agonist on LS \u03b14(3)\u03b22(2) nAChRs and \u03b16-containing subtypes as well.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/02698811221132214","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T03:13:28Z","timestamp":1667358808000},"page":"1280-1293","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Pharmacological characterization of 5-iodo-A-85380, a \u03b22-selective nicotinic receptor agonist, in mice"],"prefix":"10.1177","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0168-4515","authenticated-orcid":false,"given":"Lois S","family":"Akinola","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deniz","family":"Bagdas","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine, Yale University, New Haven, CT, USA"},{"name":"Yale Tobacco Center of Regulatory Science, Yale University, New Haven, CT, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasmin","family":"Alkhlaif","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asti","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine, Yale University, New Haven, CT, USA"},{"name":"Yale Tobacco Center of Regulatory Science, Yale University, New Haven, CT, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5327-2523","authenticated-orcid":false,"given":"Cenk O","family":"Gurdap","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Department of Women\u2019s and Children\u2019s Health, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elnaz","family":"Rahimpour","sequence":"additional","affiliation":[{"name":"Yale Tobacco Center of Regulatory Science, Yale University, New Haven, CT, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. 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Imad","family":"Damaj","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Translational Research Initiative for Pain and Neuropathy, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"bibr1-02698811221132214","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.86.5.726"},{"key":"bibr2-02698811221132214","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci9100244"},{"key":"bibr3-02698811221132214","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(03)45007-3"},{"key":"bibr4-02698811221132214","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13090"},{"key":"bibr5-02698811221132214","first-page":"42","volume":"7","author":"Atwell L","year":"1978","journal-title":"Lab 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