{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T05:27:02Z","timestamp":1784179622470,"version":"3.55.0"},"reference-count":37,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:p>\n                    We investigated the effect of isoflurane and ketamine-xylazine anesthesia on the positron emission tomography (PET) tracer [\n                    <jats:sup>18<\/jats:sup>\n                    F]SynVesT-1 in the mouse brain. [\n                    <jats:sup>18<\/jats:sup>\n                    F]SynVesT-1 PET scans were performed in C57BL\/6J mice in five conditions: isoflurane anesthesia (ANISO), ketamine-xylazine (ANKX), awake freely moving (AW), awake followed by isoflurane administration (AW\/ANISO) or followed by ketamine-xylazine (AW\/ANKX) 20\u2009min post tracer injection. ANISO, ANKX and AW scans were also performed in mice administered with levetiracetam (LEV, 200\u2009mg\/kg) to assess non-displaceable binding. Metabolite analysis was performed in ANISO, ANKX and AW mice. Finally, in vivo autoradiography in ANISO, ANKX and AW mice at 30\u2009min post-injection was performed for validation. Kinetic modeling, with a metabolite corrected image derived input function, was performed to calculate total and non-displaceable volume of distribution ( V\n                    <jats:sub>T(IDIF)<\/jats:sub>\n                    ). V\n                    <jats:sub>T(IDIF)<\/jats:sub>\n                    was higher in ANISO compared to AW (p\u2009&lt;\u20090.0001) while V\n                    <jats:sub>T(IDIF)<\/jats:sub>\n                    in ANKX was lower compared with AW (p\u2009&lt;\u20090.0001). Non-displaceable V\n                    <jats:sub>T(IDIF)<\/jats:sub>\n                    was significantly different between ANISO and AW, but not between ANKX and AW. Change in the TAC washout was observed after administration of either isoflurane or ketamine-xylazine. Observed changes in tracer kinetics and volume of distribution might be explained by physiological changes due to anesthesia, as well as by induced cellular effects.\n                  <\/jats:p>","DOI":"10.1177\/0271678x231173185","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T01:51:47Z","timestamp":1682646707000},"page":"1612-1624","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Isoflurane and ketamine-xylazine modify pharmacokinetics of [\n                    <sup>18<\/sup>\n                    F]SynVesT-1 in the mouse brain"],"prefix":"10.1177","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5381-015X","authenticated-orcid":false,"given":"Alan","family":"Miranda","sequence":"first","affiliation":[{"name":"Molecular Imaging Center Antwerp, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4205-5432","authenticated-orcid":false,"given":"Daniele","family":"Bertoglio","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center Antwerp, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caro","family":"De Weerdt","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center Antwerp, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Staelens","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center Antwerp, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen","family":"Verhaeghe","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center Antwerp, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,4,27]]},"reference":[{"key":"bibr1-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X19864081"},{"key":"bibr2-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.120.249144"},{"key":"bibr3-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-020-05149-3"},{"key":"bibr4-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1111\/epi.16653"},{"key":"bibr5-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2018.1836"},{"key":"bibr6-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-020-01567-9"},{"key":"bibr7-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-53176-8_29"},{"key":"bibr8-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1258\/la.2012.011173"},{"key":"bibr9-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.753977"},{"key":"bibr10-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.118.218669"},{"key":"bibr11-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X221101648"},{"key":"bibr12-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1109\/TMI.2017.2667889"},{"key":"bibr13-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1088\/2057-1976\/ab8c13"},{"key":"bibr14-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1109\/TMI.2019.2962237"},{"key":"bibr15-0271678X231173185","unstructured":"Angelis G, Bickell M, Kyme A, et\u00a0al. Calculated attenuation correction for awake small animal brain PET studies. In:\n                      2013 IEEE Nuclear Science Symposium and Medical Imaging Conference (2013 NSS\/MIC)\n                      . Seoul, South Korea. 2013."},{"key":"bibr16-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1186\/s13550-020-00721-2"},{"key":"bibr17-0271678X231173185","volume-title":"Model selection and multimodel inference: a practical information-theoretic approach","author":"Burnham KP","year":"2003"},{"key":"bibr18-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.281.5.H1938"},{"key":"bibr19-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1258\/la.2010.009085"},{"key":"bibr20-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048383"},{"key":"bibr21-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2021.109094"},{"key":"bibr22-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2018.00516"},{"key":"bibr23-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.089102"},{"key":"bibr24-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23169260"},{"key":"bibr25-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.864514"},{"key":"bibr26-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20946198"},{"key":"bibr27-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1186\/s13550-021-00787-6"},{"key":"bibr28-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2008.06443.x"},{"key":"bibr29-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050752"},{"key":"bibr30-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00902"},{"key":"bibr31-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2019.183140"},{"key":"bibr32-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.106.1.67"},{"key":"bibr33-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(02)03257-0"},{"key":"bibr34-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2016.09.004"},{"key":"bibr35-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.7b00627"},{"key":"bibr36-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.117.078360"},{"key":"bibr37-0271678X231173185","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/58.9.1022"}],"container-title":["Journal of Cerebral Blood Flow &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0271678X231173185","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0271678X231173185","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0271678X231173185","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:14:31Z","timestamp":1777493671000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0271678X231173185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,27]]},"references-count":37,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1177\/0271678X231173185"],"URL":"https:\/\/doi.org\/10.1177\/0271678x231173185","relation":{},"ISSN":["0271-678X","1559-7016"],"issn-type":[{"value":"0271-678X","type":"print"},{"value":"1559-7016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,27]]}}}