{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:34:09Z","timestamp":1785515649135,"version":"3.56.0"},"reference-count":68,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Ischemic stroke occurs abruptly causing sudden neurologic deficits, and therefore, very little is known about hemodynamic perturbations in the brain immediately after stroke onset. Here, functional ultrasound imaging was used to monitor variations in relative cerebral blood volume (rCBV) compared to baseline. rCBV levels were analyzed brain-wide and continuously at high spatiotemporal resolution (100\u2009\u03bcm, 2\u2009Hz) until 70mins after stroke onset in rats. We compared two stroke models, with either a permanent occlusion of the middle cerebral artery (MCAo) or a tandem occlusion of both the common carotid and middle cerebral arteries (CCAo\u2009+\u2009MCAo). We observed a typical hemodynamic pattern, including a quick drop of the rCBV after MCAo, followed by spontaneous reperfusion of several brain regions located in the vicinity of the ischemic core. The severity and location of the ischemia were variable within groups. On average, the severity of the ischemia was in good agreement with the lesion volume (24\u2009hrs after stroke) for MCAo group, while larger for the CCAo\u2009+\u2009MCAo model. For both groups, we observed that infarcts extended to initially non-ischemic regions located rostrally to the ischemic core. These regions strongly colocalize with the origin of transient hemodynamic events associated with spreading depolarizations.<\/jats:p>","DOI":"10.1177\/0271678x231191600","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T07:17:03Z","timestamp":1690528623000},"page":"6-18","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Brain-wide continuous functional ultrasound imaging for real-time monitoring of hemodynamics during ischemic stroke"],"prefix":"10.1177","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2567-4832","authenticated-orcid":false,"given":"Cl\u00e9ment","family":"Brunner","sequence":"first","affiliation":[{"name":"Neuro-Electronics Research Flanders, Leuven, Belgium"},{"name":"Vlaams Instituut voor Biotechnologie, Leuven, Belgium"},{"name":"Interuniversity Microelectronics Centre, Leuven, Belgium"},{"name":"Department of Neurosciences, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nielsen Lagumersindez","family":"Denis","sequence":"additional","affiliation":[{"name":"Department of Neurology and Center for Stroke Research Berlin, Charit\u00e9-Universit\u00e4tsmedizin, Berlin, Germany"},{"name":"German Center for Neurodegenerative Diseases (DZNE), Berlin, Germany"},{"name":"German Centre for Cardiovascular Research (DZHK), Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Gertz","sequence":"additional","affiliation":[{"name":"Department of Neurology and Center for Stroke Research Berlin, Charit\u00e9-Universit\u00e4tsmedizin, Berlin, Germany"},{"name":"German Center for Neurodegenerative Diseases (DZNE), Berlin, Germany"},{"name":"German Centre for Cardiovascular Research (DZHK), Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micheline","family":"Grillet","sequence":"additional","affiliation":[{"name":"Neuro-Electronics Research Flanders, Leuven, Belgium"},{"name":"Vlaams Instituut voor Biotechnologie, Leuven, Belgium"},{"name":"Interuniversity Microelectronics Centre, Leuven, Belgium"},{"name":"Department of Neurosciences, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Montaldo","sequence":"additional","affiliation":[{"name":"Neuro-Electronics Research Flanders, Leuven, Belgium"},{"name":"Vlaams Instituut voor Biotechnologie, Leuven, Belgium"},{"name":"Interuniversity Microelectronics Centre, Leuven, Belgium"},{"name":"Department of Neurosciences, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Endres","sequence":"additional","affiliation":[{"name":"Department of Neurology and Center for Stroke Research Berlin, Charit\u00e9-Universit\u00e4tsmedizin, Berlin, Germany"},{"name":"German Center for Neurodegenerative Diseases (DZNE), Berlin, Germany"},{"name":"German Centre for Cardiovascular Research (DZHK), Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Urban","sequence":"additional","affiliation":[{"name":"Neuro-Electronics Research Flanders, Leuven, Belgium"},{"name":"Vlaams Instituut voor Biotechnologie, Leuven, Belgium"},{"name":"Interuniversity Microelectronics Centre, Leuven, Belgium"},{"name":"Department of Neurosciences, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,7,28]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.12.6.723"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.109.552513"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000196957.55928.ab"},{"key":"e_1_3_4_5_2","first-page":"147","volume-title":"Neuromethods","author":"Mart\u00edn A","year":"2016","unstructured":"Mart\u00edn A, Ramos-Cabrer P, Planas AM. Noninvasive brain imaging in small animal stroke models: MRI, PET, and SPECT. In: Neuromethods. New York: Springer, 2016, pp.147\u2013186."},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0000000000000275"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2017.07.018"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-021-01575-5"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2014.06.063"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1641"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1109\/TUFFC.2013.2592"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-111020-100706"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X15622466"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X18786359"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.44233"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2333"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00027.2014"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199306000-00027"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199611000-00002"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199603000-00004"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01769-2"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.02.007"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.121.038085"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1988.88"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01398.x"},{"key":"e_1_3_4_26_2","first-page":"3445","article-title":"Animal models of ischemic stroke and their application in clinical research","volume":"9","author":"Fluri F","year":"2015","unstructured":"Fluri F, Schuhmann MK, Kleinschnitz C. Animal models of ischemic stroke and their application in clinical research. Drug Des Devel Ther 2015; 9: 3445\u20133454.","journal-title":"Drug Des Devel Ther"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2020.09.020"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-021-00548-8"},{"key":"e_1_3_4_29_2","volume-title":"The rat brain in stereotaxic coordinates","author":"Paxinos G.","year":"2014","unstructured":"Paxinos G. The rat brain in stereotaxic coordinates. 6th ed. Amsterdam: Academic Press, 2014.","edition":"6"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_3_4_31_2","first-page":"53106","article-title":"Performing permanent distal middle cerebral with common carotid artery occlusion in aged rats to study cortical ischemia with sustained disability","volume":"108","author":"Wayman C","year":"2016","unstructured":"Wayman C, Duricki DA, Roy LA, et\u00a0al. Performing permanent distal middle cerebral with common carotid artery occlusion in aged rats to study cortical ischemia with sustained disability. J Vis Exp 2016; 108: 53106.","journal-title":"J Vis Exp"},{"key":"e_1_3_4_32_2","unstructured":"ClemBrunner nerf-common. Nerf-common\/whole-brain-fUS: Whole-brain-fUS. Zenodo 2021. doi:10.5281\/ZENODO.4905923."},{"key":"e_1_3_4_33_2","first-page":"007","article-title":"Functional ultrasound imaging of cerebral capillaries in rodents and humans","volume":"1","author":"Urban A","year":"2015","unstructured":"Urban A, Brunner C, Dussaux C, et\u00a0al. Functional ultrasound imaging of cerebral capillaries in rodents and humans. Jacobs J Mol Transl Med 2015; 1: 007.","journal-title":"Jacobs J Mol Transl Med"},{"key":"e_1_3_4_34_2","unstructured":"ClemBrunner nerf-common. nerf-common\/whole-brain-fUS: whole-brain-fUS v1.0. 2021. doi:10.5281\/zenodo.4585348."},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01284-4"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0572-y"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.07.014"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050083"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.29.12.2522"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.17.4.738"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1052-3057(99)80045-7"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13231-016-0015-0"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16688705"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awq117"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.62"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2014.136"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600569"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3694-15.2016"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1917-10.2010"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.3171\/foc.1996.1.3.1"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X15625578"},{"key":"e_1_3_4_53_2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1097\/00132586-198404000-00002","article-title":"Cerebral protection by isoflurane during hypoxemia or ischemia","volume":"28","author":"Newberg LA","year":"1984","unstructured":"Newberg LA, Michenfelder JD, Chalon J. Cerebral protection by isoflurane during hypoxemia or ischemia. Surv Anesthesiol 1984; 28: 83.","journal-title":"Surv Anesthesiol"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199708000-00004"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-37-11602.2003"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2009.06812.x"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnene.2014.00001"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2016.03.049"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.4103\/2045-9912.254640"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.11.031"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2021.04.008"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.02.012"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16774-9"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4147-07.2008"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3408"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awy102"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X18793324"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.108.541128"},{"key":"e_1_3_4_69_2","doi-asserted-by":"crossref","unstructured":"Brunner C Montaldo G Urban A. Functional ultrasound imaging of stroke in awake rats. 2023. DOI: 10.7554\/elife.88919.1.","DOI":"10.7554\/eLife.88919.1"}],"container-title":["Journal of Cerebral Blood Flow &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0271678X231191600","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0271678X231191600","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0271678X231191600","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:14:37Z","timestamp":1777493677000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0271678X231191600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,28]]},"references-count":68,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1177\/0271678X231191600"],"URL":"https:\/\/doi.org\/10.1177\/0271678x231191600","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.01.19.476904","asserted-by":"object"}]},"ISSN":["0271-678X","1559-7016"],"issn-type":[{"value":"0271-678X","type":"print"},{"value":"1559-7016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,28]]}}}