{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T16:54:40Z","timestamp":1789232080412,"version":"build-2803163510"},"reference-count":21,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:p>\n                    Current techniques for inducing intraluminal filamentous middle cerebral artery occlusion (fMCAo) in mice produce highly variable results and often cause additional infarcts in the posterior cerebral artery (PCA) territory. The aim of the current study was to develop a novel procedure to overcome these shortcomings. Male C57BL\/6 mice were subjected to 60\u2009min of fMCAo with cerebral blood flow monitored by laser Doppler flowmetry. The influence of the length of the occlusion filament coating and the combination of common carotid artery (CCA) or pterygopalatine artery (PPA) ligation on lesion volume and functional outcome 24\u2009h after reperfusion was evaluated. The use of appropriate filament and PPA ligation while maintaining CCA perfusion prevented the development of infarcts in the PCA area, resulted in pure MCA infarcts (68.3\u2009\u00b1\u200914.5\u2009mm\n                    <jats:sup>3<\/jats:sup>\n                    ) and reduced the variability of infarct volumes by more than half (from 26\u201338% to 14% standard deviation\/mean). Using an improved fMCAo procedure, we were able to produce\n                    <jats:underline>P<\/jats:underline>\n                    CA area-\n                    <jats:underline>u<\/jats:underline>\n                    naffected\n                    <jats:underline>re<\/jats:underline>\n                    producible (PURE) infarcts exclusively in the MCA territory. Thus PURE-MCAo reduced outcome variability by more than 50%. Our results may thus help to reduce the number of animals in preclinical stroke research and to increase the reproducibility of the fMCAo model.\n                  <\/jats:p>","DOI":"10.1177\/0271678x241281841","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T05:24:49Z","timestamp":1728278689000},"page":"871-884","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Reliable infarction of the middle cerebral artery territory in C57BL\/6 mice using pterygopalatine artery ligation and filament optimization \u2013 The PURE-MCAo model"],"prefix":"10.1177","volume":"45","author":[{"given":"Sodai","family":"Yoshimura","sequence":"first","affiliation":[{"name":"Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University Munich (LMU), Germany"},{"name":"Department of Neurosurgery, Nihon University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3895-8401","authenticated-orcid":false,"given":"Maximilian","family":"Dorok","sequence":"additional","affiliation":[{"name":"Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University Munich (LMU), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uta","family":"Mamrak","sequence":"additional","affiliation":[{"name":"Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University Munich (LMU), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Wehn","sequence":"additional","affiliation":[{"name":"Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University Munich (LMU), Germany"},{"name":"Department of Neurosurgery, LMU University Hospital, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Krestel","sequence":"additional","affiliation":[{"name":"Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University Munich (LMU), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1041-5747","authenticated-orcid":false,"given":"Igor","family":"Khalin","sequence":"additional","affiliation":[{"name":"Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University Munich (LMU), Germany"},{"name":"Normandie University, UNICAEN, INSERM UMR-S U1237, Physiopathology and Imaging of Neurological Disorders (PhIND), GIP Cyceron, Institute Blood and Brain @ Caen-Normandie (BB@C), Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaus","family":"Plesnila","sequence":"additional","affiliation":[{"name":"Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University Munich (LMU), Germany"},{"name":"Munich Cluster for Systems Neurology (Synergy), Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa9853"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3995\/jstroke.8.1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.20.1.84"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.122.039337"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.1998.0235"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199805000-00012"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.112.653204"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(03)02300-X"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3171\/2011.11.JNS111167"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2011.2105"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2008.06.021"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1990.47"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.17.3.472"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148503"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.576741"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.114.007832"},{"key":"e_1_3_2_18_2","first-page":"111","article-title":"Carotid artery occlusion and collateral circulation in C57Black\/6J mice detected by synchrotron radiation microangiography","volume":"52","author":"Tamaki M","year":"2006","unstructured":"Tamaki M, Kidoguchi K, Mizobe T, et\u00a0al. Carotid artery occlusion and collateral circulation in C57Black\/6J mice detected by synchrotron radiation microangiography. 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