{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T17:37:16Z","timestamp":1787679436299,"version":"build-2784847793"},"reference-count":54,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:p>\n                    Glucose metabolic dysfunction is a hallmark of Alzheimer\u2019s disease (AD) pathology and is used to diagnose the disease or predict imminent cognitive decline. The main method to measure brain metabolism\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    is positron emission tomography with 2-Deoxy-2-[\n                    <jats:sup>18<\/jats:sup>\n                    F]fluoroglucose ([\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG-PET). The cellular origin of changes in the [\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG-PET signal in AD is controversial. We addressed this by combining [\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG-PET with subsequent cell-sorting and \u03b3-counting of [\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG-accumulation in sorted cell populations. 7-month-old male TgF344-AD rats and wild-type controls (n\u2009=\u200924\/group) received sham or ceftriaxone (200\u2009mg\/kg) injection prior to [\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG-PET imaging to increase glutamate uptake and glucose utilisation. The same animals were injected again one week later, and radiolabelled brains were dissected, with hippocampi taken for magnetically-activated cell sorting of radioligand-treated tissues (MACS-RTT). Radioactivity in sorted cell populations was measured to quantify cell-specific [\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG uptake. Transcriptional analyses of metabolic enzymes\/transporters were also performed.\n                    <jats:italic toggle=\"yes\">Hypo<\/jats:italic>\n                    metabolism in the frontal association cortex of TgF344-AD rats was identified using [\n                    <jats:sup>18<\/jats:sup>\n                    F]FDG-PET, whereas\n                    <jats:italic toggle=\"yes\">hyper<\/jats:italic>\n                    metabolism was identified in the hippocampus using MACS-RTT. Hypermetabolism was primarily driven by GLAST+ cells. This was supported by transcriptional analyses which showed alteration to metabolic apparatus, including upregulation of hexokinase 2 and altered expression of glucose\/lactate transporters. See Figure 1 for summary.\n                  <\/jats:p>","DOI":"10.1177\/0271678x251318923","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T05:08:23Z","timestamp":1738904903000},"page":"1326-1342","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Early metabolic changes in the brain of Alzheimer\u2019s disease rats are driven by GLAST+ cells"],"prefix":"10.1177","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7994-1166","authenticated-orcid":false,"given":"William J","family":"Morrey","sequence":"first","affiliation":[{"name":"Division of Neuroscience, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK"},{"name":"Geoffrey Jefferson Brain Research Centre, Manchester Academic Health Science Centre, Northern Care Alliance &amp; University of Manchester, Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Ceyz\u00e9riat","sequence":"additional","affiliation":[{"name":"CIBM Center for BioMedical Imaging, Geneva, Switzerland"},{"name":"Department of Psychiatry, University of Geneva, Geneva, Switzerland"},{"name":"Department of Psychiatry, University Hospitals of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quentin","family":"Amoss\u00e9","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of Geneva, Geneva, Switzerland"},{"name":"Department of Psychiatry, University Hospitals of Geneva, Geneva, Switzerland"},{"name":"University of Lausanne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1890-8986","authenticated-orcid":false,"given":"Aur\u00e9lien M","family":"Badina","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5018-2111","authenticated-orcid":false,"given":"Ben","family":"Dickie","sequence":"additional","affiliation":[{"name":"Geoffrey Jefferson Brain Research Centre, Manchester Academic Health Science Centre, Northern Care Alliance &amp; 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