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Limited evidence exists regarding the systematic comparison of their pathophysiological characteristics, particularly considering the age of experimental mice. In this study, cerebral infarction outcomes were analyzed in mice aged 3, 6, 9, and 12 months. Mice aged 3\u20136 months exhibited minor cerebral infarction with KMCAO but substantial infarction with LMCAO, whereas differences diminished in 9\u201312-month-old mice. Comparable infarct severity in 3-month-old mice was achieved by inducing ischemia for 1.5 hours using KMCAO and for 1 hour using LMCAO, suggesting potential mechanistic similarities, subject to further research. LMCAO led to reperfusion injury due to preserved CCA perfusion, whereas KMCAO provided controlled ischemic insult via continuous CCA ligation. Both models confirmed that increased brain damage correlated with decreased neuronal nuclei expression and increased glial fibrillary acidic protein expression. These findings highlight the importance of selecting the MCAO model based on experimental objectives, age, and ischemia duration for the accurate modeling of ischemic and degenerative brain injury.<\/jats:p>","DOI":"10.1177\/0271678x251352691","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T11:28:38Z","timestamp":1750937318000},"page":"2189-2202","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessing the effects of aging on the Koizumi\u2019s and Zea Longa\u2019s methods and their suitability as mouse models for evaluating neurodegeneration post-ischemic stroke: A comparative study"],"prefix":"10.1177","volume":"45","author":[{"given":"Chiyeon","family":"Lim","sequence":"first","affiliation":[{"name":"Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongrae","family":"Kim","sequence":"additional","affiliation":[{"name":"Pusan National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sehyun","family":"Lim","sequence":"additional","affiliation":[{"name":"Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, USA"},{"name":"Department of Nursing, School of Public Health, Far East University, Eumseong, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo-Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Pusan National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So-Jung","family":"Moon","sequence":"additional","affiliation":[{"name":"Integrated Science and Engineering Division, Underwood International College, Yonsei University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung-Hwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8507-1382","authenticated-orcid":false,"given":"Suin","family":"Cho","sequence":"additional","affiliation":[{"name":"Pusan National University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,26]]},"reference":[{"key":"e_1_3_4_2_2","first-page":"29","article-title":"Heart disease and stroke statistics\u20132015 update: a report from the American heart association","volume":"131","author":"Mozaffarian D","year":"2015","unstructured":"Mozaffarian D, Benjamin EJ, Go AS, et\u00a0al. 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