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However, the temporal evolution of cerebral A\n                    <jats:sub>2<\/jats:sub>\n                    ARs density after cerebral ischemia and its subsequent neuroinflammatory response have been scarcely explored. In this study, the expression of A\n                    <jats:sub>2<\/jats:sub>\n                    ARs after transient middle cerebral artery occlusion (MCAO) was evaluated in rats by positron emission tomography (PET) with [\n                    <jats:sup>11<\/jats:sup>\n                    C]SCH442416 and immunohistochemistry (IHC). In addition, the role of A\n                    <jats:sub>2<\/jats:sub>\n                    ARs in stroke inflammation with pharmacological modulation was assessed with magnetic resonance imaging (MRI), PET imaging with [\n                    <jats:sup>18<\/jats:sup>\n                    F]DPA-714 (TSPO), IHC, western-blot, and autoradiography. After cerebral ischemia, [\n                    <jats:sup>11<\/jats:sup>\n                    C]SCH442416 and IHC revealed neural expression of A\n                    <jats:sub>2<\/jats:sub>\n                    ARs in the striatum in healthy brains, followed by a binding decrease at day 1 and a subsequent significant increase at day 3 after ischemia in microglia and infiltrated leukocytes. Furthermore, activation of A\n                    <jats:sub>2<\/jats:sub>\n                    ARs with the agonist CGS-21680 resulted in a reduction in stroke volume, along with an increase in TSPO expression in immune cells in the striatum. Our results provide novel evidence on A\n                    <jats:sub>2<\/jats:sub>\n                    ARs density dynamics after cerebral ischemia that might guide the therapeutic management of stroke by modulating adenosine receptors.\n                  <\/jats:p>","DOI":"10.1177\/0271678x251370835","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T09:47:17Z","timestamp":1757324837000},"page":"456-472","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Longitudinal imaging evaluation of the inflammatory role of purinergic A\n                    <sub>2<\/sub>\n                    A receptors during subacute and chronic ischemic stroke"],"prefix":"10.1177","volume":"46","author":[{"given":"Maider","family":"Garbizu","sequence":"first","affiliation":[{"name":"Achucarro Basque Center for Neuroscience, Leioa, Spain"},{"name":"CIC biomaGUNE, Basque Research and Technology 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