{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T06:32:32Z","timestamp":1780986752927,"version":"3.54.1"},"reference-count":54,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"national natural science foundation of china","doi-asserted-by":"publisher","award":["82371308, 82130035, 82401521, 82101399"],"award-info":[{"award-number":["82371308, 82130035, 82401521, 82101399"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Sun Yat-sen University Clinical Research 5010 Program","award":["2018001"],"award-info":[{"award-number":["2018001"]}]},{"name":"Guangdong Provincial Clinical Research Center for Neurological Diseases","award":["2020B1111170002"],"award-info":[{"award-number":["2020B1111170002"]}]},{"name":"Guangdong Provincial Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases","award":["2020B1212060017"],"award-info":[{"award-number":["2020B1212060017"]}]},{"name":"Guangdong Province International Cooperation Base for Early Intervention and Functional Rehabilitation of Neurological Diseases","award":["2015B050501003 and 2020A0505020004"],"award-info":[{"award-number":["2015B050501003 and 2020A0505020004"]}]},{"name":"Guangzhou Clinical Research and Translational Center for Major Neurological Diseases","award":["(201604020010"],"award-info":[{"award-number":["(201604020010"]}]},{"name":"Guangdong Provincial Engineering Center for Major Neurological Disease Treatment"},{"DOI":"10.13039\/100016101","name":"Guangdong Provincial Translational Medicine Innovation Platform for Diagnosis and Treatment of Major Neurological Disease","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016101","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:p>\n                    Glucose-6-phosphate dehydrogenase (G6PD) deficiency, the most common hereditary enzyme defect in the world, may be involved in cerebrovascular abnormalities suggested by clinical research. However, whether and how G6PD deficiency impairs cerebral vasculature is poorly understood. We therefore aimed at determining the role of G6PD in brain vasculature. By evaluating cerebrovascular imaging data from stroke patients, we found patients with G6PD deficiency exhibited greater morphological variability in the circle of Willis (CoW) with reduced frequency of the anterior communicating artery, bilateral A1 segments, but a higher incidence of fetal posterior cerebral artery (fPCA). To further confirm G6PD deficiency was associated with cerebrovascular abnormalities, we used pharmacological inhibitor to suppress G6PD activity in zebrafish embryos and utilized CRISPR\/Cas9 technology to generate\n                    <jats:italic toggle=\"yes\">g6pd<\/jats:italic>\n                    homozygous knockout and endothelial cell-specific knockout zebrafish lines. We found all of these models demonstrated varying degrees of cerebrovascular morphological abnormalities and impairment of cerebrovascular angiogenesis. Transcriptomic data analysis and in vitro experiments further elucidated that G6PD deficiency led to dysfunctional angiogenic response and induced apoptosis. Altogether, we first demonstrated that G6PD deficiency may be associated with CoW variants in stroke patients and impairing cerebrovascular morphology and angiogenesis during zebrafish development.\n                  <\/jats:p>","DOI":"10.1177\/0271678x251377678","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T10:55:15Z","timestamp":1758797715000},"page":"738-752","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["G6PD deficiency is implicated in Circle of Willis variants in stroke patients and impairs brain vasculature in zebrafish"],"prefix":"10.1177","volume":"46","author":[{"given":"Yuangui","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China"},{"name":"Guangdong Provincial Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, Guangzhou, 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