{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T14:18:12Z","timestamp":1793110692098,"version":"4.5.0"},"reference-count":50,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Robert E. and Donna Landreth Fund for the Study of Neuroimmune Interactions in Autism"},{"name":"The Intricate Knot Foundation"},{"name":"national institutes of health","award":["S10RR017208, S10RR023401, S10OD023517"],"award-info":[{"award-number":["S10RR017208, S10RR023401, S10OD023517"]}]},{"name":"national institutes of health","award":["S10RR026666, S10RR0229, S10RR01993376,"],"award-info":[{"award-number":["S10RR026666, S10RR0229, S10RR01993376,"]}]},{"name":"massachusetts life sciences center"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>\n                    Cerebral folate receptor \u03b1 (FR\u03b1) is essential for transporting folate into the central nervous system, supporting normal biological functions and fetal neurodevelopment. We aimed to provide\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    measurements of folic acid dynamics at FR\u03b1 in non-human primate brains using positron emission tomography (PET) and [\n                    <jats:sup>18<\/jats:sup>\n                    F]AzaFol, a high-affinity FR\u03b1 radioligand. A 0.01\u2009mg\/kg intravenous dose of folic acid, comparable to pregnancy supplementation doses (400\u2013800\u2009\u00b5g), dramatically reduced FR\u03b1 availability in the choroid plexus (CP), based on uptake profiles and exploratory kinetic modeling. Following this dose, [\n                    <jats:sup>18<\/jats:sup>\n                    F]AzaFol uptake increased, potentially capturing (1) FR\u03b1-mediated transport of folic acid and (2) real-time FR\u03b1 recycling, which may restore binding sites as serum folic acid levels decline, reducing receptor competition. Experiments with constant infusion of folic acid during PET imaging aimed to identify the possible folic acid transport rate and serum concentrations at which this process reaches a steady state. Pharmacokinetic analysis suggests full FR\u03b1 blockage with serum folic acid concentrations within the range of 63\u201390\u2009ng\/mL and cumulative dose slightly above 0.01\u2009mg\/kg. Findings enhance the understanding of folic acid\u2019s\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    pharmacology at cerebral FR\u03b1 and may inform future studies on disorders in which impaired folic acid transport is suspected, either at the CP or at the placenta.\n                  <\/jats:p>","DOI":"10.1177\/0271678x251399065","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T05:23:45Z","timestamp":1764566625000},"page":"976-986","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["[\n                    <sup>18<\/sup>\n                    F]AzaFol PET captures folic acid dynamics at folate receptors in the brain"],"prefix":"10.1177","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5136-4494","authenticated-orcid":false,"given":"Frederick A","family":"Bagdasarian","sequence":"first","affiliation":[{"name":"Athinoula A. 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Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, SC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph W","family":"Downey","sequence":"additional","affiliation":[{"name":"Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, SC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob M","family":"Hooker","sequence":"additional","affiliation":[{"name":"Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, SC, USA"},{"name":"Lurie Center for Autism, Massachusetts General Hospital, Lexington, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole R","family":"Z\u00fcrcher","sequence":"additional","affiliation":[{"name":"Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, SC, USA"},{"name":"Lurie Center for Autism, Massachusetts General Hospital, Lexington, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1425-8489","authenticated-orcid":false,"given":"Hsiao-Ying","family":"Wey","sequence":"additional","affiliation":[{"name":"Athinoula A. 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