{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:34:12Z","timestamp":1792787652101,"version":"4.5.0"},"reference-count":33,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100004587","name":"instituto de salud carlos iii","doi-asserted-by":"publisher","award":["PI22\/01016 and RD24\/0007\/0027"],"award-info":[{"award-number":["PI22\/01016 and RD24\/0007\/0027"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union","id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]},{"name":"DeckTherapeutics, Inc"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Acute injection of omega-3 fatty acids (n-3) formulated in a novel diglyceride (DG) lipid emulsion is neuroprotective after hypoxic-ischemic encephalopathy (HIE) in neonatal rodents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      The efficacy of acute n-3 DG emulsion treatment was tested in 2 to 3-day-old piglets subjected to bilateral carotid artery clamping and 8% O\n                      <jats:sub>2<\/jats:sub>\n                      exposure for 30\u2009min. Animals were randomly assigned to normothermia or hypothermia (HT, 34\u2009\u00b1\u20090.5\u00b0C), then to n-3 DG or vehicle administration 30\u2009min post-HI. Brain activity was assessed by amplitude-integrated EEG over 6\u2009h. Brain injury was analyzed by TUNEL, GFAP and IBA-1 staining, \u00b9H-NMR spectroscopy (Lactate\/NAA, Glutamate\/NAA), and Western blot (caspase-3, TNF-\u03b1, IL-1\u03b2, protein carbonylation).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>n-3 DG was well tolerated, with no hemodynamic or respiratory side effects. HT alone moderately improved brain activity and only reduced inflammation, whereas n-3 DG treatment partially restored early EEG recovery, attenuated cell death and neuroinflammatory markers, and limited protein carbonyl formation and GFAP+ cell loss. Notably, n-3 DG efficacy was preserved under HT conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>These results support the neuroprotective potential of n-3 DG emulsion in a piglet model of HIE, with promising implications for clinical translation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0271678x261494381","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:05:58Z","timestamp":1790229958000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Neuroprotective efficacy of an omega-3 diglyceride emulsion in a piglet model of newborn hypoxic-ischemic brain damage"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4442-4879","authenticated-orcid":false,"given":"Luis","family":"Arruza","sequence":"first","affiliation":[{"name":"Department of Neonatology Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"},{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Romero","sequence":"additional","affiliation":[{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"De Hoz","sequence":"additional","affiliation":[{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Silva","sequence":"additional","affiliation":[{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Martinez-Vega","sequence":"additional","affiliation":[{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Neonatology Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"},{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niki","family":"Oikonomopoulou","sequence":"additional","affiliation":[{"name":"Department of Neonatology Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"},{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Vierge","sequence":"additional","affiliation":[{"name":"Department of Neonatology Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"},{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0333-6242","authenticated-orcid":false,"given":"Esther","family":"Aleo","sequence":"additional","affiliation":[{"name":"Biomedical Research Foundation, Hospital Ni\u00f1o Jes\u00fas-FIBHNJS, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Ramos-Corral","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Hospital Cl\u00ednico San Carlos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purificaci\u00f3n","family":"Jurado-Parras","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Hospital Cl\u00ednico San Carlos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hylde","family":"Zirpoli","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Deckelbaum","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4405-3545","authenticated-orcid":false,"given":"Jos\u00e9","family":"Martinez-Orgado","sequence":"additional","affiliation":[{"name":"Department of Neonatology Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"},{"name":"Biomedical Research Foundation, Hospital Cl\u00ednico San Carlos - IdISSC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2013.206"},{"key":"e_1_3_5_3_2","first-page":"99","article-title":"Current and emerging therapies in the management of hypoxic ischemic encephalopathy in neonates","volume":"5","author":"Nair J","year":"2018","unstructured":"Nair J, Kumar VHS. Current and emerging therapies in the management of hypoxic ischemic encephalopathy in neonates. Children (Basel) 2018; 5: 99.","journal-title":"Children (Basel)"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2021.05.014"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2013.10.005"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.21037\/tp-23-253"},{"key":"e_1_3_5_7_2","unstructured":"Sakr M Balasundaram P. Neonatal therapeutic hypothermia. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing. https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK567714\/ (2022 accessed 7 October 2025)."},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0000000000000199"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/archpediatrics.2011.1772"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2023.12152"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(21)00264-3"},{"key":"e_1_3_5_12_2","article-title":"Omega-3 fatty acids for the primary and secondary prevention of cardiovascular disease","volume":"7","author":"Abdelhamid AS","year":"2018","unstructured":"Abdelhamid AS, Brown TJ, Brainard JS, et al. Omega-3 fatty acids for the primary and secondary prevention of cardiovascular disease. Cochrane Database Syst Rev 2018; 7: Cd003177.","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.117.014092"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23105390"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-36653-9"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-082018-124539"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116749"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e3182276b11"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e318186e5dd"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2018.11.020"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0201895"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1515\/jpm-2017-0355"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1515\/jpm-2016-0334"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116274"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2020.102176"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.618419"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep23430"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70016-3"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004410000203"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.01.010"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2009.07.009"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12101839"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160870"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1159\/000496602"}],"container-title":["Journal of Cerebral Blood Flow &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0271678X261494381","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0271678X261494381","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0271678X261494381","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:19:28Z","timestamp":1792153168000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0271678X261494381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"references-count":33,"alternative-id":["10.1177\/0271678X261494381"],"URL":"https:\/\/doi.org\/10.1177\/0271678x261494381","relation":{},"ISSN":["0271-678X","1559-7016"],"issn-type":[{"value":"0271-678X","type":"print"},{"value":"1559-7016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]},"article-number":"0271678X261494381"}}