{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:33:44Z","timestamp":1777509224247,"version":"3.51.4"},"reference-count":38,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:00:00Z","timestamp":1698019200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571131"],"award-info":[{"award-number":["81571131"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Acta Radiol"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The middle cerebral artery occlusion model (MCAo) is a commonly used animal model for cerebral ischemia studies but lacks accessible imaging techniques for the assessment of hemodynamic changes of the model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>The study aims to explore the value of contrast-enhanced ultrasound (CEUS) in evaluating brain perfusion in the early stages after MCAo surgery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>\n                      In total, 18 adult male Sprague-Dawley rats were subjected to right MCAo using an intraluminal filament model, and CEUS was performed at the three following timepoints: before (T0), immediately after (T1), and 6\u2005h after permanent MCAo (T2). Twelve rats successfully completed the study, and their brains were removed and stained using 2, 3, 5-triphenyltetrazolium chloride (TTC). CEUS video images were visualized offline, and the time-intensity curves (TICs) were analyzed. Different cerebrovascular patterns and manifestations of the contrast enhancement in rat ischemic hemispheres were observed. Semi-quantitative parameters of TICs in ischemic areas (ROI\n                      <jats:sub>i<\/jats:sub>\n                      ) and the surrounding normal- or hypo-perfused areas (ROI\n                      <jats:sub>n<\/jats:sub>\n                      ) were calculated and compared between T0, T1, and T2, and also between ROI\n                      <jats:sub>i<\/jats:sub>\n                      and ROI\n                      <jats:sub>n<\/jats:sub>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A significant correlation was found between the lesion volume (%) determined by TTC and CEUS parameters (r\u2009=\u2009\u22120.691, P\u2009=\u20090.013 for peak intensity; r\u2009=\u2009\u22120.742, P\u2009=\u20090.006 for area under the curve) at T2. After the same occlusion, there were differences in contrast perfusion in each group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study suggests that CEUS could be an effective imaging tool for studying cerebral ischemia and perfusion in small animals as long as the transcranial acoustic window allows it.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/02841851231205163","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T23:40:33Z","timestamp":1698104433000},"page":"3042-3051","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic assessment of brain perfusion in a middle cerebral artery occlusion rat model by contrast-enhanced ultrasound imaging: a pilot study"],"prefix":"10.1177","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0661-9384","authenticated-orcid":false,"given":"Chenyun","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, Sichuan, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxia","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, Sichuan, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7073-6150","authenticated-orcid":false,"given":"Yan","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, Sichuan, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, Sichuan, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,10,23]]},"reference":[{"key":"bibr1-02841851231205163","doi-asserted-by":"publisher","DOI":"10.2217\/fnl.14.44"},{"key":"bibr2-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2016.02.016"},{"key":"bibr3-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2017.09.022"},{"key":"bibr4-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.24.2.294"},{"key":"bibr5-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2990.2000.00221.x"},{"key":"bibr6-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.29.10.2162"},{"key":"bibr7-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.2017.1375661"},{"key":"bibr8-02841851231205163","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2013.1046"},{"key":"bibr9-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075779"},{"key":"bibr10-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2010.10"},{"key":"bibr11-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2011.12.006"},{"key":"bibr12-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148503"},{"key":"bibr13-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16661567"},{"key":"bibr14-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0320-7_7"},{"key":"bibr15-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1111\/ijs.12186"},{"key":"bibr16-02841851231205163","volume":"145","author":"Kaspar M","year":"2015","journal-title":"Swiss Med Wkly"},{"key":"bibr17-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-010-1537-7"},{"key":"bibr18-02841851231205163","doi-asserted-by":"publisher","DOI":"10.7863\/ultra.34.2.179"},{"key":"bibr19-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1159\/000366237"},{"key":"bibr20-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1097\/RLI.0000000000000188"},{"key":"bibr21-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1159\/000484635"},{"key":"bibr22-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1038\/nature16066"},{"key":"bibr23-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2015.09.037"},{"key":"bibr24-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.20.1.84"},{"key":"bibr25-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16657574"},{"key":"bibr26-02841851231205163","first-page":"1441","volume":"21","author":"Mayer TE","year":"2000","journal-title":"Am J Neuroradiol"},{"key":"bibr27-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000114877.58809.3D"},{"key":"bibr28-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1990.47"},{"key":"bibr29-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1177\/1747493017701942"},{"key":"bibr30-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2016.09.002"},{"key":"bibr31-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1136\/neurintsurg-2016-012628"},{"key":"bibr32-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurad.2016.12.009"},{"key":"bibr33-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1007\/s00234-017-1914-z"},{"key":"bibr34-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.14"},{"key":"bibr35-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2017.08.935"},{"key":"bibr36-02841851231205163","doi-asserted-by":"publisher","DOI":"10.3171\/2015.11.FOCUS15570"},{"key":"bibr37-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1227\/NEU.0000000000000301"},{"key":"bibr38-02841851231205163","doi-asserted-by":"publisher","DOI":"10.1007\/s00247-018-4270-1"}],"container-title":["Acta Radiologica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/02841851231205163","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/02841851231205163","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/02841851231205163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:33:44Z","timestamp":1777390424000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.1177\/02841851231205163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,23]]},"references-count":38,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1177\/02841851231205163"],"URL":"https:\/\/doi.org\/10.1177\/02841851231205163","relation":{},"ISSN":["0284-1851","1600-0455"],"issn-type":[{"value":"0284-1851","type":"print"},{"value":"1600-0455","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,23]]}}}