{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:24:30Z","timestamp":1777728270592,"version":"3.51.4"},"reference-count":13,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2013,1,23]],"date-time":"2013-01-23T00:00:00Z","timestamp":1358899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2013,2]]},"abstract":"\n Objective<\/jats:title>\n Seroprevalence surveys of healthcare workers for vaccine-preventable diseases, including measles and varicella, are essential for disease prevention and infection control programmes. The purpose of this study was to compare the complement fixation (CF) assay and an enzyme immunoassay (EIA) to determine the prevalence of immunoglobulin G antibodies directed against measles and varicella viruses in healthcare workers.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Antimeasles and antivaricella antibody titres were measured simultaneously in serum samples from healthcare workers employed at a Japanese university hospital, using the CF assay and an EIA.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Serum samples were obtained from 898 healthcare workers. Seropositivity rates determined using the CF assay and EIA were 67.8% versus 94.0%, respectively, for measles, and 83.2% versus 97.6% for varicella. Compared with EIA, a nine- and 22-fold higher number of seronegative subjects was identified by the CF assay for measles and varicella, respectively.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Differences between the CF assay and EIA in detecting seronegative or seropositive healthcare workers for measles and varicella suggest that undertaking a seroprevalence survey using an EIA, rather than a CF assay, would more accurately determine susceptibility to vaccine-preventable diseases, in healthcare settings.<\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/0300060512474773","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T04:57:04Z","timestamp":1359003424000},"page":"224-230","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative analysis of a complement fixation assay and enzyme immunoassay to determine the seroprevalence of measles and varicella in a survey of healthcare workers"],"prefix":"10.1177","volume":"41","author":[{"given":"Hiroshi","family":"Shibata","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunichi","family":"Kumakura","sequence":"additional","affiliation":[{"name":"Department of Medical Education and Research, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Isobe","sequence":"additional","affiliation":[{"name":"Division of Clinical Oncology and Respiratory Medicine, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Hirose","sequence":"additional","affiliation":[{"name":"Centre for Education on Hospital Medicine, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miki","family":"Ohe","sequence":"additional","affiliation":[{"name":"Department of Medical Education and Research, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiro","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Pharmacy Department, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiichi","family":"Onoda","sequence":"additional","affiliation":[{"name":"Third Division of Internal Medicine, Faculty of Medicine, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Nagai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhei","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Third Division of Internal Medicine, Faculty of Medicine, Shimane University, Izumo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,1,23]]},"reference":[{"key":"bibr1-0300060512474773","first-page":"1","volume":"46","author":"Immunization of health-care workers","year":"1997","journal-title":"MMWR Recomm Rep"},{"key":"bibr2-0300060512474773","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/jcm.9.6.657-664.1979","volume":"9","author":"Forghani B","year":"1979","journal-title":"J Clin Microbiol"},{"key":"bibr3-0300060512474773","first-page":"71","volume":"53","author":"Baumgarten A","year":"1980","journal-title":"Yale J Biol Med"},{"key":"bibr4-0300060512474773","unstructured":"Centers for Disease Control and Prevention (CDC): Progress toward measles elimination, Japan, 1999\u20132008.\n MMWR Morb Mortal Wkly Rep\n 2008; 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