{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:35:12Z","timestamp":1791048912019,"version":"4.1.0"},"reference-count":19,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2013,3,7]],"date-time":"2013-03-07T00:00:00Z","timestamp":1362614400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To develop and evaluate a novel system for detecting and quantifying hepatitis A virus (HAV) nucleic acid.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Real-time TaqMan\u00ae reverse transcription\u2013polymerase chain reaction (PCR) procedures were established, based on amplification of the highly conserved 5\u2032-non-coding region. Synthetic single-stranded RNA transcripts synthesized in\u00a0vitro were used as the quantification standard. Ten-fold dilutions were prepared from HAV strain stock suspension to determine precision, accuracy, sensitivity and specificity. In addition, serum specimens from patients with acute HAV underwent clinical evaluation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The novel assay had a detection limit for HAV RNA of 10 TCID\n                      <jats:sub>50<\/jats:sub>\n                      \/ml (where TCID\n                      <jats:sub>50<\/jats:sub>\n                      is median tissue culture infective dose). It was more sensitive and specific than the commercial quantitative PCR kit manufactured by Shanghai Zhijiang Bio-Tech. However, the Artus HAV RT\u2013PCR kit (Qiagen) had the best performance of the three assays and had a detection limit of 5 TCID\n                      <jats:sub>50<\/jats:sub>\n                      \/ml. The new HAV real-time PCR detection system was also successfully applied in 90 serum specimens from patients with confirmed acute HAV infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Considering its high reproducibility, sensitivity, specificity and simplicity, this novel amplification system may be suitable for wide clinical application as a diagnostic tool, and for the surveillance and investigation of infectious diseases in developing countries where HAV is endemic.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060513476434","type":"journal-article","created":{"date-parts":[[2013,3,7]],"date-time":"2013-03-07T20:17:37Z","timestamp":1362687457000},"page":"427-434","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative evaluation of a novel TaqMan real-time reverse transcription\u2013polymerase chain reaction assay for hepatitis A virus detection"],"prefix":"10.1177","volume":"41","author":[{"given":"Feng","family":"Qiu","sequence":"first","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Yi","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Jia","sequence":"additional","affiliation":[{"name":"Institute 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