{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:27:21Z","timestamp":1777728441298,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2013,8,9]],"date-time":"2013-08-09T00:00:00Z","timestamp":1376006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the influence of recombinant human CD40 ligand (rhCD40L) on the biological behaviour of breast cancer cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      MDA-MB-23l and MDA-MB-435 treated with rhCD40L were observed for changes in the cell cycle, in membrane proteins, and in mRNA levels of B cell lymphoma-extra-large (Bcl-xl), Bcl-2 associated X protein (Bax) and regulated upon activation, normal T cell expressed and secreted (RANTES). Effects of rhCD40L on cell proliferation in the presence or absence of interferon (IFN)\n                      <jats:bold>-<\/jats:bold>\n                      \u03b3 (500\u2009IU\/ml) and\/or doxorubicin (20\u2009ng\/ml) were also determined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      rhCD40L dose-dependently inhibited cell proliferation. Combination of rhCD40L with IFN-\u03b3 or doxorubicin potentiated the inhibitory activity. After treatment, an increase in cells entering the G\n                      <jats:sub>1<\/jats:sub>\n                      phase of the cell cycle was observed, with a significant decrease in the number entering the S phase. Levels of several membrane proteins including CD95L and CD120a were also increased. Reverse transcription\u2013polymerase chain reaction revealed an increase in the Bax\/Bcl-xl mRNA ratio and an increase in RANTES.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>rhCD40L treatment of breast cancer cells mediates a variety of anti-tumour effects, not only by direct cytotoxic activity but also by upregulation of adhesion molecules, co-stimulators and cytokines to rectify T cell immunity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060513490084","type":"journal-article","created":{"date-parts":[[2013,8,9]],"date-time":"2013-08-09T23:59:15Z","timestamp":1376092755000},"page":"1495-1504","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of CD40 ligation combined with chemotherapy drugs on human breast cancer cell lines"],"prefix":"10.1177","volume":"41","author":[{"given":"Zhi-Qing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Emergency Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Emergency Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Hua","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Emergency Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-Guang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Clinical Immunology Centre, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,8,9]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.22828"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201242552"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.149"},{"key":"e_1_3_3_5_2","first-page":"69","article-title":"CD40\/CD40 ligand interactions in immune responses and pulmonary immunity","volume":"73","author":"Kawabe T","year":"2011","unstructured":"Kawabe T, Matsushima M, Hashimoto N, et al. CD40\/CD40 ligand interactions in immune responses and pulmonary immunity. Nagoya J Med Sci 2011; 73: 69\u201378.","journal-title":"Nagoya J Med Sci"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0612266"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/14653249.2011.605296"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1206954"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-04-351254"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2011.605190"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2004.02.008"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3109\/08830185.2012.698338"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.1.41.41"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.159.12.5782"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.1.162"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V124695"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.4.2214"},{"key":"e_1_3_3_18_2","first-page":"1909","article-title":"CD11a-CD18 and CD102 interactions mediate human myeloma cell growth arrest induced by CD40 stimulation","volume":"56","author":"Pellat-Deceunynck C","year":"1996","unstructured":"Pellat-Deceunynck C, Amiot M, Robillard N, et al. CD11a-CD18 and CD102 interactions mediate human myeloma cell growth arrest induced by CD40 stimulation. Cancer Res 1996; 56: 1909\u20131916.","journal-title":"Cancer Res"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2012.03.003"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90509-O"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202568"},{"key":"e_1_3_3_22_2","first-page":"664","article-title":"Millimeter wave radiation induces apoptosis via affecting the ratio of Bax\/Bcl-2 in SW1353 human chondrosarcoma cells","volume":"27","author":"Li X","year":"2012","unstructured":"Li X, Ye H, Cai L, et al. Millimeter wave radiation induces apoptosis via affecting the ratio of Bax\/Bcl-2 in SW1353 human chondrosarcoma cells. Oncol Rep 2012; 27: 664\u2013672.","journal-title":"Oncol Rep"}],"container-title":["Journal of International Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300060513490084","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0300060513490084","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300060513490084","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:52:06Z","timestamp":1777459926000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0300060513490084"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,9]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1177\/0300060513490084"],"URL":"https:\/\/doi.org\/10.1177\/0300060513490084","relation":{},"ISSN":["0300-0605","1473-2300"],"issn-type":[{"value":"0300-0605","type":"print"},{"value":"1473-2300","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,9]]}}}