{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T14:45:25Z","timestamp":1789397125228,"version":"build-2803163510"},"reference-count":18,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2013,10]]},"abstract":"\n Objective<\/jats:title>\n \n To test the proof-of-principle that genetically-engineered mesenchymal stem cells (MSCs) transfected with the human hyperpolarization-activated cyclic nucleotide-gated channel 1 (\n hHCN1<\/jats:italic>\n ) gene can be modified to become cardiac pacemaker cells.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n MSCs were transfected with the\n hHCN1<\/jats:italic>\n gene using lentiviral-based transfection. The expressed pacemaker current (\n I<\/jats:italic>\n f<\/jats:sub>\n ) in\n hHCN1<\/jats:italic>\n -transfected MSCs was recorded using whole-cell patch-clamp analysis. The effect of the\n hHCN1<\/jats:italic>\n -transfected MSCs on cardiomyocyte excitability was determined by coculturing the MSCs with neonatal rabbit ventricular myocytes (NRVM). The spontaneous action potentials of the NRVM were recorded by whole-cell current-clamp analysis.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A high level time- and voltage-dependent inward hyperpolarization current that was inhibited by 4\u2009mM caesium chloride was detected in\n hHCN1<\/jats:italic>\n -transfected MSCs, suggesting that the HCN1 proteins acted as\n I<\/jats:italic>\n f<\/jats:sub>\n channels in MSCs. The mean\u2009\u00b1\u2009SE beating frequency in NRVMs cocultured with control MSCs transfected with the pcDNA3 plasmid control was 82\u2009\u00b1\u20098 beats\/min (\n n<\/jats:italic>\n \u2009=\u20095) compared with 129\u2009\u00b1\u200911 beats\/min (\n n<\/jats:italic>\n \u2009=\u20095) in NRVMs cocultured with\n hHCN1<\/jats:italic>\n -transfected MSCs.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Genetically-engineered MSCs transfected with the\n hHCN1<\/jats:italic>\n gene can be modified to become cardiac pacemaker cells.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/0300060513501123","type":"journal-article","created":{"date-parts":[[2013,10,4]],"date-time":"2013-10-04T09:34:10Z","timestamp":1380879250000},"page":"1570-1576","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Genetically-engineered mesenchymal stem cells transfected with human\n HCN1<\/i>\n gene to create cardiac pacemaker cells"],"prefix":"10.1177","volume":"41","author":[{"given":"Ya-Feng","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Xia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian-Huan","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Ping","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,10,4]]},"reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2011.09.005"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00029.2008"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/092986711796642427"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8159.2009.02614.x"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/147323000803600523"},{"key":"e_1_3_1_7_2","first-page":"1571","article-title":"Overexpression of connexin 45 in rat mesenchymal stem cells improves their function as cardiac biological pacemaker","volume":"123","author":"Tong M","year":"2010","unstructured":"Tong M, Yang XJ, Geng BY, et al. 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