{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:30:56Z","timestamp":1777728656341,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T00:00:00Z","timestamp":1435881600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To explore the role of Na\n                      <jats:sup>+<\/jats:sup>\n                      \/H\n                      <jats:sup>+<\/jats:sup>\n                      exchanger regulatory factor 1 (NHERF1) in autosomal-dominant polycystic kidney disease (ADPKD).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>NHERF1 and \u03b2-catenin protein were detected by immunohistochemistry and Western blotting of kidney tissue samples from patients with ADPKD and controls (normal kidney tissue [&gt;5\u2009cm from the foci] collected from patients undergoing unilateral nephrectomy for kidney cancer). NHERF1 and \u03b2-catenin protein and mRNA were quantified by Western blot and real-time fluorescent quantitative polymerase chain reaction, respectively, in kidney tissue samples from Han:SPRD (+\/+) and (cy\/+) rats. The effects of human recombinant NHERF1 on proliferation and cell cycle of ADPKD cyst-lining epithelial cells (WT9\u201312) were evaluated by MTT assay and flow cytometry, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Levels of NHERF1 protein and mRNA were significantly lower, and \u03b2-catenin levels significantly higher, in patients with ADPKD and Han:SPRD (cy\/+) rats, compared with control subjects and (+\/+) rats, respectively. Exogenous recombinant NHERF1 significantly inhibited proliferation of WT9\u201312 cells and increased the proportion of cells in G\n                      <jats:sub>0<\/jats:sub>\n                      \/G\n                      <jats:sub>1<\/jats:sub>\n                      phase.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ADPKD is associated with a decrease in NHERF1 protein and mRNA levels. Supplementing exogenous NHERF1 inhibited the proliferation of WT9\u201312 cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060515581182","type":"journal-article","created":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T21:40:15Z","timestamp":1435959615000},"page":"629-638","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Expression of Na\n                    <sup>+<\/sup>\n                    \/H\n                    <sup>+<\/sup>\n                    exchanger regulatory factor 1 in autosomal-dominant polycystic kidney disease"],"prefix":"10.1177","volume":"43","author":[{"given":"Jian-Qing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Nephrology, Second Affiliated Hospital of Zhejiang University, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, First People\u2019s Hospital of Yuhang District, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Second Affiliated Hospital of Zhejiang University, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,7,3]]},"reference":[{"key":"bibr1-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfs551"},{"key":"bibr2-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2013.160"},{"key":"bibr3-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20609"},{"key":"bibr4-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.02.010"},{"key":"bibr5-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn208"},{"key":"bibr6-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M410697200"},{"key":"bibr7-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702019200"},{"key":"bibr8-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-07-0617"},{"key":"bibr9-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2007.02730.x"},{"key":"bibr10-0300060515581182","doi-asserted-by":"publisher","DOI":"10.3892\/or.2012.1895"},{"key":"bibr11-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-015-9848-3"},{"key":"bibr12-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1186\/bcr3067"},{"key":"bibr13-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00310.2002"},{"key":"bibr14-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1997.276"},{"key":"bibr15-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60601-1"},{"issue":"2","key":"bibr16-0300060515581182","first-page":"F210","volume":"276","author":"Sorenson CM","year":"1999","journal-title":"Am J Physiol"},{"key":"bibr17-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204825"},{"key":"bibr18-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1159\/000298962"},{"key":"bibr19-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2003.50270"},{"key":"bibr20-0300060515581182","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600979"},{"key":"bibr21-0300060515581182","first-page":"295","volume":"19","author":"Wilson J","year":"1993","journal-title":"J Oral Implantol"},{"key":"bibr22-0300060515581182","doi-asserted-by":"publisher","DOI":"10.2174\/156652408785748031"}],"container-title":["Journal of International Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300060515581182","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0300060515581182","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300060515581182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:52:47Z","timestamp":1777459967000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0300060515581182"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,3]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1177\/0300060515581182"],"URL":"https:\/\/doi.org\/10.1177\/0300060515581182","relation":{},"ISSN":["0300-0605","1473-2300"],"issn-type":[{"value":"0300-0605","type":"print"},{"value":"1473-2300","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,3]]}}}