{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:32:55Z","timestamp":1777728775048,"version":"3.51.4"},"reference-count":38,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T00:00:00Z","timestamp":1474934400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate whether circulating cell-free (cf)-DNA levels are a useful biomarker for survival in patients with shock in the emergency intensive care unit (EICU).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This prospective observational study enrolled patients admitted to the EICU diagnosed with shock. Blood cf-DNA levels were analysed on admission, and after 24 and 48\u2009h. As a measure of circulating cf-DNA, copy number of the \u03b2-globin gene in plasma was assessed using quantitative real-time polymerase chain reaction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Circulating cf-DNA levels were higher at hospital admission and after 24\u2009h in EICU patients with shock who died than in those who recovered. Change in cf-DNA levels over the first 48\u2009h in critical care was independently associated with 28-day mortality. The critical cut-off value for cf-DNA change over 48\u2009h in predicting 28-day mortality was +16.12% (sensitivity 68.9%, specificity 89.7%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Increased circulating cf-DNA levels in EICU patients with shock are associated with risk of death and measuring cf-DNA change over 48\u2009h improves risk prediction. The present study suggests that cf-DNA may serve as a viable plasma biomarker of mortality risk in EICU patients with shock.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060516650785","type":"journal-article","created":{"date-parts":[[2016,7,7]],"date-time":"2016-07-07T22:49:02Z","timestamp":1467931742000},"page":"1002-1012","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Cell-free DNA increase over first 48 hours in emergency intensive care unit predicts fatal outcome in patients with shock"],"prefix":"10.1177","volume":"44","author":[{"given":"Dun Ling","family":"Xia","sequence":"first","affiliation":[{"name":"Emergency Department, First Affiliated Hospital of Anhui Medical University, Hefei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Emergency Department, First Affiliated Hospital of 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