{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:34:39Z","timestamp":1777728879301,"version":"3.51.4"},"reference-count":35,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T00:00:00Z","timestamp":1484179200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Cutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Novy\u2013MacNeal\u2013Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal transcribed spacer 1 (ITS1) of the Leishmania genome in cultures containing Leishmania promastigotes from 100 patients, which were genotyped with a restriction fragment length polymorphism (RFLP) analysis. A phylogenetic tree was constructed from ITS1 sequences of 95 culture fluid samples from these patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Leishmania amastigotes were detected in 92% of cultures with growth. Leishmania promastigotes were typed as Leishmania tropica with both PCR\u2013RFLP and sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Identification of L. tropica as the causative agent of CL in our region allows the clinical course to be predicted, and guides treatment decisions and preventive measures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060516677155","type":"journal-article","created":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T04:31:40Z","timestamp":1484195500000},"page":"114-122","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Genotyping\n                    <i>Leishmania<\/i>\n                    promastigotes isolated from patients with cutaneous leishmaniasis in south-eastern Turkey"],"prefix":"10.1177","volume":"45","author":[{"given":"Fahriye","family":"Ek\u015fi","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orhan","family":"\u00d6zg\u00f6zta\u015f\u0131","sequence":"additional","affiliation":[{"name":"Department of Skin and Venereal Diseases, Gaziantep University, Gaziantep, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tekin","family":"Karsl\u0131gil","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa","family":"Sa\u011flam","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,1,12]]},"reference":[{"key":"bibr1-0300060516677155","first-page":"198","volume-title":"T\u0131bbi parazit hastaliklari 1","author":"\u00d6zbel Y","year":"2007"},{"key":"bibr2-0300060516677155","doi-asserted-by":"publisher","DOI":"10.5152\/tpd.2012.29"},{"key":"bibr3-0300060516677155","unstructured":"UNHCR\u2009-\u2009BM M\u00fclteciler Y\u00fcksek Komiserli\u011fi (www.unhcr.org.tr)."},{"key":"bibr4-0300060516677155","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.73.999"},{"key":"bibr5-0300060516677155","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2010.09.003"},{"key":"bibr6-0300060516677155","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.12.001"},{"key":"bibr7-0300060516677155","doi-asserted-by":"publisher","DOI":"10.1007\/s40257-015-0114-z"},{"key":"bibr8-0300060516677155","doi-asserted-by":"publisher","DOI":"10.5402\/2013\/308726"},{"key":"bibr9-0300060516677155","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2009.02384.x"},{"key":"bibr10-0300060516677155","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2000.63.43"},{"key":"bibr11-0300060516677155","unstructured":"\u00d6zbel Y and T\u00f6z SO. Leishmaniasis. In: Korkmaz M and Ok \u00dcZ (ed)\n                      Parazitolojide laboratuar\n                      . T\u00fcrkiye Parazitoloji Derne\u011fi, Yay\u0131n No:23, 2011, pp.307\u2013320."},{"key":"bibr12-0300060516677155","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2014.09.014"},{"key":"bibr13-0300060516677155","unstructured":"Manual molecular procedures. Training course Molecular Epidemiology Leishmaniasis. Rio de Janeiro Brazil: Instituto Oswaldo Cruz, 2009, pp. 27\u201332. Available at: http:\/\/clioc.fiocruz.br\/documents\/mmp.pdf."},{"key":"bibr14-0300060516677155","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(00)90093-2"},{"key":"bibr15-0300060516677155","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbn013"},{"key":"bibr16-0300060516677155","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2010.03851.x"},{"key":"bibr17-0300060516677155","unstructured":"WHO. 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