{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:39:12Z","timestamp":1777729152251,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T00:00:00Z","timestamp":1504137600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the vasodilative and endothelial-protective effects and the underlying mechanisms of total flavonoids from Astragalus (TFA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The vasodilative activities of TFA were measured with a myograph ex vivo using rat superior mesenteric arterial rings. The primary human umbilical vein endothelial cell (HUVEC) viabilities were assayed using the cell counting kit-8 after hypoxia or normoxia treatment with or without TFA. Akt, P-Akt, eNOS, P-eNOS, Erk, P-Erk, Bcl-2 and Bax expression were analyzed using western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>TFA showed concentration-dependent vasodilative effects on rat superior mesenteric arterial rings, but had no effects on normal or potassium chloride precontracted arterial rings. TFA did not affect HUVEC viabilities in normoxia, but dramatically promoted cell proliferation in the concentration range of 1 to 30\u2009\u00b5g\/mL under hypoxia. Moreover, TFA significantly increased the ratios of P-Akt\/Akt and P-eNOS\/eNOS in vascular endothelial cells under hypoxic conditions, but did not change the P-Erk\/Erk or Bcl-2\/Bax ratios.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>TFA might exhibit vasorelaxant and endothelial-protective effects via the Akt\/eNOS signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060517717358","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T08:13:23Z","timestamp":1504167203000},"page":"2096-2103","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Total flavonoids from\n                    <i>Astragalus<\/i>\n                    alleviate endothelial dysfunction by activating the Akt\/eNOS pathway"],"prefix":"10.1177","volume":"46","author":[{"given":"Qian","family":"Liu","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiyuan","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxia","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaocai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meifei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanjie","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Medicine, Akron General Medical Center, Ohio, 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