{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T03:19:57Z","timestamp":1785727197167,"version":"3.56.0"},"reference-count":32,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T00:00:00Z","timestamp":1515628800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the clinical value of human papillomavirus (HPV) E6 and E7 oncoprotein (HPV E6\/E7) detection in the early screening of cervical cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This prospective study evaluated all patients with suspected cervical intraepithelial neoplasia (CIN) as identified by the presence of at least one positive indicator from a ThinPrep cytologic test (TCT) and\/or a Hybrid Capture 2 (HC2) HPV DNA test. The levels of E6\/E7 oncoproteins were determined using Western blot analysis. The diagnostic value of the HPV E6\/E7 protein assay was compared with the clinical diagnosis from TCT, HC2 and the gold standard of cervical biopsy histology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 450 patients were enrolled in the study and based on histological findings, 102 patients were diagnosed with CIN1 (22.7%), 241 with CIN2 (53.6%), 96 with CIN3 (21.3%) and 11 with squamous cell carcinoma (2.4%). For a diagnosis of CIN2+, although the sensitivity of the HPV E6\/E7 assay was lower than HC2 (65.5% versus 96.6%, respectively), the specificity was higher (38.2% versus 5.9%, respectively). The sensitivity of the HPV E6\/E7 assay was higher than TCT (65.5% versus 36.2%, respectively).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Measuring HPV E6\/E7 oncoprotein levels is a potential new biomarker for HPV type 16.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060517736913","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T02:15:05Z","timestamp":1515723305000},"page":"1033-1042","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Feasibility study of a human papillomavirus E6 and E7 oncoprotein test for the diagnosis of cervical precancer and cancer"],"prefix":"10.1177","volume":"46","author":[{"given":"Jin-Jun","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Henan Province Chinese Medicine Hospital, Zhengzhou, Henan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Chun","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Henan Province Chinese Medicine Hospital, Zhengzhou, Henan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Yu","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Henan Province Chinese Medicine Hospital, Zhengzhou, Henan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6415-2308","authenticated-orcid":false,"given":"Hai-Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Henan Province Chinese Medicine Hospital, Zhengzhou, Henan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Henan Province Chinese Medicine Hospital, Zhengzhou, Henan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,1,11]]},"reference":[{"key":"bibr1-0300060517736913","doi-asserted-by":"publisher","DOI":"10.21106\/ijma.12"},{"key":"bibr2-0300060517736913","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2012.03.024"},{"key":"bibr3-0300060517736913","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgp263"},{"key":"bibr4-0300060517736913","first-page":"1149","volume":"12","author":"Li J","year":"2011","journal-title":"Asian Pac J Cancer Prev"},{"key":"bibr5-0300060517736913","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28982"},{"key":"bibr6-0300060517736913","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-27-36"},{"key":"bibr7-0300060517736913","doi-asserted-by":"publisher","DOI":"10.2741\/1971"},{"key":"bibr8-0300060517736913","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1438.2005.00246.x"},{"key":"bibr9-0300060517736913","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdx220"},{"key":"bibr10-0300060517736913","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-012-2697-4"},{"key":"bibr11-0300060517736913","unstructured":"World Health Organization. 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