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Locations of 3-mercaptopyruvate sulfurtransferase (3-MPST) and cysteine aminotransferase (CAT) were examined immunohistochemically. Levels of H\n                      <jats:sub>2<\/jats:sub>\n                      S, eNOS, phosphorylated-eNOS\n                      <jats:sup>ser1177<\/jats:sup>\n                      , and PDE5A were measured.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In IMA segments from 47 patients, acetylcholine-induced relaxation (resistant to N\n                      <jats:sup>G<\/jats:sup>\n                      -nitro-L-arginine and indomethacin) was significantly attenuated by aminooxyacetic acid or L-aspartate (CAT inhibitors), iberiotoxin (large-conductance calcium-activated K\n                      <jats:sup>+<\/jats:sup>\n                      channel blocker), TRAM-34 plus apamin (intermediate- and small-conductance Ca\n                      <jats:sup>2+<\/jats:sup>\n                      -activated K\n                      <jats:sup>+<\/jats:sup>\n                      channel blockers) or glibenclamide (ATP-sensitive K\n                      <jats:sup>+<\/jats:sup>\n                      channel blocker). 3-MPST and mitochondrial CAT were found in endothelial and smooth muscle cells while cytosolic CAT was located only in endothelial cells. Acetylcholine significantly increased the H\n                      <jats:sub>2<\/jats:sub>\n                      S levels. The H\n                      <jats:sub>2<\/jats:sub>\n                      S donor, NaHS, increased eNOS phosphorylation and down-regulated PDE5A.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Human conduit artery endothelium releases H\n                      <jats:sub>2<\/jats:sub>\n                      S under basal and stimulated conditions, involving the 3-MPST\/CAT pathway, eNOS phosphorylation, PDE5A activity, and potassium channels. These findings may provide new therapeutic targets for treating vasospasm in CABG grafts and facilitate the development of new vasodilator drugs.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060519847386","type":"journal-article","created":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T02:54:41Z","timestamp":1559530481000},"page":"3778-3791","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Hydrogen sulfide-mediated endothelial function and the interaction with eNOS and PDE5A activity in human internal mammary arteries"],"prefix":"10.1177","volume":"47","author":[{"given":"Chao","family":"Yuan","sequence":"first","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"},{"name":"Postdoctoral Station, Medical College, Nankai University, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Tao","family":"Hou","sequence":"additional","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"},{"name":"Medical College, Zhejiang University, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan-Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng-Qing","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tie-Nan","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Novakovic","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marija","family":"Marinko","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6497-876X","authenticated-orcid":false,"given":"Guo-Wei","family":"He","sequence":"additional","affiliation":[{"name":"Center for Basic Medical Research and Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China"},{"name":"Medical College, Zhejiang University, Hangzhou, China"},{"name":"School of Pharmacy, Wannan Medical College, Wuhu, Anhui, China"},{"name":"Department of Surgery, Oregon Health and Science University, Portland, Oregon, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,6,2]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1202916109"},{"key":"e_1_3_3_3_2","first-page":"4","article-title":"Hydrogen sulfide (H2S) - the third gas of interest for pharmacologists.","volume":"59","author":"\u0141owicka E","year":"2007","unstructured":"\u0141owicka E, Be\u0142towski J. 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