{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:20:32Z","timestamp":1777728032329,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T00:00:00Z","timestamp":1576627200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008111","name":"Hainan Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["(SQ2016SHFZ0044)"],"award-info":[{"award-number":["(SQ2016SHFZ0044)"]}],"id":[{"id":"10.13039\/501100008111","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the Th1\/Th2 cell profile in spleens of cirrhotic and hypersplenic rats by investigating the expression of Th1-associated chemokine receptors CXCR3, CCR5 and Th2-associated chemokine receptor CCR3.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Experimental liver cirrhosis and hypersplenism were induced in rats by the intragastric administration of carbon tetrachloride (CCl\n                      <jats:sub>4<\/jats:sub>\n                      ; 40% solution [0.3 ml\/100g, twice\/week for 8 weeks]) and confirmed by pathology and hemogram. Presence of the three chemokine receptors was investigated by real-time polymerase chain reaction (RT-PCR), immunohistochemical staining, and western blot analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>By comparison with control animals (n=10), RT-PCR demonstrated that CXCR3 and CCR5-mRNA levels were significantly elevated in the hypersplenic rats (n=26) and CCR3-mRNA levels were lower. Immunohistochemical staining showed that by comparison with controls, the mean density of the Th1-associated CXCR3 and CCR5 receptors was significantly increased but there was no difference between groups in Th2-associated CCR3 receptors. Western blot analysis showed that by comparison with controls, hypersplenic rats had higher levels of CXCR3 and CCR5 protein but lower levels of CCR3 protein.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The abnormal expression of Th1-associated chemokine receptors in spleens of rats with cirrhosis and hypersplenism induced by CCL4 suggests that a functional imbalance between Th1\/Th2 cells may play a role in the pathogenesis of hypersplenism.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060519889441","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T02:14:16Z","timestamp":1576721656000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Investigation into imbalance of Th1\/Th2 cells in cirrhotic, hypersplenic rats"],"prefix":"10.1177","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0451-399X","authenticated-orcid":false,"given":"Yunfu","family":"Lv","sequence":"first","affiliation":[{"name":"Department of General Surgery, Hainan General Hospital, Haikou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yejuan","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Medical Centre, Hainan Women and Children's Medical Centre, Haikou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Hainan General Hospital, Haikou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonghong","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Shanxi Provincial People's Hospital, Haikou 030001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Hainan General Hospital, Haikou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,12,18]]},"reference":[{"key":"bibr1-0300060519889441","first-page":"17","volume":"27","author":"Fang JJ","year":"2014","journal-title":"Biomed Environ Sci"},{"key":"bibr2-0300060519889441","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3683"},{"key":"bibr3-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aau6085"},{"key":"bibr4-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-017-9487-9_3"},{"key":"bibr5-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2011.0065"},{"key":"bibr6-0300060519889441","unstructured":"CAO Zhixin, CHEN Xiaoping, WU Zaide. A study on the spleen Th1\/Th2 cytokines immunological condition in the patients with primary hepatocellular carcinoma combined with liver cirrhosis.\n                      Chin J Exp Surg\n                      2001\u20132006. http:\/\/en.cnki.com.cn\/Article_en\/CJFDTOTAL-ZHSY200106016.htm"},{"key":"bibr7-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1038\/34814"},{"key":"bibr8-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-508044"},{"key":"bibr9-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2011.03485.x"},{"key":"bibr10-0300060519889441","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00277"},{"key":"bibr11-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1111\/liv.12472"},{"key":"bibr12-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1111\/hepr.12352"},{"key":"bibr13-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096043"},{"key":"bibr14-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2008.08.003"},{"key":"bibr15-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1080\/10590500701569398"},{"key":"bibr16-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(13)60115-7"},{"key":"bibr17-0300060519889441","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-014-0321-x"},{"key":"bibr18-0300060519889441","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v7.i11.1572"},{"key":"bibr19-0300060519889441","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2015.1016218"},{"key":"bibr20-0300060519889441","doi-asserted-by":"publisher","DOI":"10.3109\/09537100903420277"}],"container-title":["Journal of International Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300060519889441","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0300060519889441","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300060519889441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:51:06Z","timestamp":1777459866000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0300060519889441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,18]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1177\/0300060519889441"],"URL":"https:\/\/doi.org\/10.1177\/0300060519889441","relation":{},"ISSN":["0300-0605","1473-2300"],"issn-type":[{"value":"0300-0605","type":"print"},{"value":"1473-2300","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,18]]},"article-number":"0300060519889441"}}