{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:48:57Z","timestamp":1777726137524,"version":"3.51.4"},"reference-count":26,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81672234"],"award-info":[{"award-number":["81672234"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shenzhen Science and Technology Project","award":["201606018"],"award-info":[{"award-number":["201606018"]}]},{"name":"the fund of Health and Family Planning Commission of Shenzhen Municipality","award":["SZBC2017022"],"award-info":[{"award-number":["SZBC2017022"]}]},{"name":"Shenzhen Science and Technology Innovation Council","award":["GCZX2015043017241191"],"award-info":[{"award-number":["GCZX2015043017241191"]}]},{"name":"Shenzhen Science and Technology Innovation Council","award":["JCYJ20150330102401096"],"award-info":[{"award-number":["JCYJ20150330102401096"]}]},{"name":"Shenzhen Science and Technology Innovation Council","award":["JCYJ20160226192924528"],"award-info":[{"award-number":["JCYJ20160226192924528"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Construction of adenoviral vectors can be complicated and time-consuming. The aim of this study was to construct an adenoviral vector expressing human bone morphogenetic protein 2 (BMP-2).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      An adenoviral vector expressing human BMP-2 was constructed using the Gateway\u2122 technique based on site-specific recombination. Briefly, BMP-2 cDNA was obtained by polymerase chain reaction, inserted into the pMD19-T cloning vector, and subcloned into a shuttle vector. The DNA sequence encoding BMP-2 was then subcloned from pEC3.1-BMP2 into the pAd\/BLOCK-iT\u2122-DEST adenoviral vector genome, which was then linearized and used to transfect 293 cells for adenovirus packaging. Adenovirus particles were generated with a titer of 1\u2009\u00d7\u200910\n                      <jats:sup>10<\/jats:sup>\n                      infectious units\/mL.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Potent expression of BMP-2 mRNA and protein was detected in adenovirus-infected 293 cells, confirming that the adenoviral vector encoding BMP-2 was successfully constructed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This recombinant adenoviral vector encoding BMP-2 can be applied in future studies to explore the roles of BMP-2 in various cell types and tissues.<\/jats:p>\n                  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