{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:53:46Z","timestamp":1777726426721,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate drug resistance features and homology among penicillin-intermediate Streptococcus pneumoniae isolates from Wenzhou City, China.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fifty-one penicillin-intermediate S. pneumoniae isolates were obtained from respiratory samples of infants and children hospitalized with lung infections. An antimicrobial susceptibility test was used to assess drug resistance. Polymerase chain reaction and agarose gel electrophoresis were used to identify S. pneumoniae isolates and pulsed-field gel electrophoresis (PFGE) was used to analyze molecular subtypes. Hierarchical cluster analysis of PFGE fingerprints was used to compare genetic diversity and relatedness of S. pneumoniae isolates. The Quellung test was used for serotyping.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Fifty-one penicillin-intermediate S. pneumoniae isolates showed evidence of multi-drug resistance and polyclonal origins. The isolates were classified into 25 subtypes through hierarchical cluster analysis of PFGE fingerprints. Three of these subtypes formed a supertype (15\/51, 16\/51 and 8\/51 isolates), while the remaining subtypes occurred sporadically (12\/51 isolates).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Transmission of penicillin-intermediate S. pneumoniae is mostly vertical and to a lesser extent horizontal. Effective prevention strategies, including respiratory tract management and contact isolation, are essential to control nosocomial S. pneumoniae infection. Once susceptibility is confirmed, vancomycin, high-dose penicillin or third-generation cephalosporins (cefotaxime and ceftriaxone) may be used to treat penicillin-intermediate S. pneumoniae.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060520919061","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T07:41:47Z","timestamp":1591947707000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Homology analysis of 51 penicillin-intermediate\n                    <i>Streptococcus pneumoniae<\/i>\n                    isolates from Wenzhou City, China"],"prefix":"10.1177","volume":"48","author":[{"given":"Jin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da-Kang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Tangshan Maternal and Child Health Hospital, Tangshan, Hebei Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Wei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Taizhou Center for Disease Control and Prevention, Taizhou, Zhejiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Hua","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang-Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Yu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology, Wuhan, Hubei Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3758-7186","authenticated-orcid":false,"given":"Wu-Shuang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Qing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Taizhou Women and Children\u2019s Hospital, Taizhou, Zhejiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,6,12]]},"reference":[{"key":"bibr1-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2013.11.026"},{"key":"bibr2-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-11-3049"},{"key":"bibr3-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.01.013"},{"key":"bibr4-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2014.11.002"},{"key":"bibr5-0300060520919061","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1128\/JCM.38.1.351-353.2000","volume":"38","author":"McEllistrem MC","year":"2000","journal-title":"J Clin Microbiol"},{"key":"bibr6-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2565-2571.2001"},{"key":"bibr7-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-012-0027-9"},{"key":"bibr8-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3095-3100.2005"},{"key":"bibr9-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01311-13"},{"key":"bibr10-0300060520919061","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2013.1688"},{"key":"bibr11-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.2.453-457.1998"},{"key":"bibr12-0300060520919061","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.31.8.2097-2100.1993"},{"key":"bibr13-0300060520919061","unstructured":"Clinical and Laboratory Standard Institute.\n                      Performance Standards for Antimicrobial Susceptibility Testing; 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