{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:53:36Z","timestamp":1777726416355,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to determine the effect of parathyroid hormone-related protein (PTHrP) on proliferation of cardiac fibroblasts (CFs) in primary cultures of neonatal Wistar rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Different PTHrP concentrations were added to CFs of neonatal Wistar rats and the cells were grouped according to the concentrations added. A verapamil (VPL) group and a calcitriol (CAL) group were also established. Changes in cell proliferation and in cyclic adenosine monophosphate and calcium ion levels were identified and recorded.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found that as the concentration of PTHrP increased, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT, a tetrazolium salt) colorimetric absorbance values (A values) decreased. These values in the PTHrP groups were significantly lower than those in the control group. MTT colorimetric A values and\n                      <jats:sup>3<\/jats:sup>\n                      H-thymidine deoxyribose intake were lower in the VPL group, low-dose CAL group, and the PTHrP 10\n                      <jats:sup>\u22127<\/jats:sup>\n                      mol\/L group compared with the control group. However, MTT colorimetric A values and\n                      <jats:sup>3<\/jats:sup>\n                      H-thymidine deoxyribose intake were higher in the high-dose CAL group than in the PTHrP 10\n                      <jats:sup>\u22127<\/jats:sup>\n                      mol\/L group. As PTHrP concentrations increased, intracellular cyclic adenosine monophosphate concentrations also increased.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PTHrp, VPL, and low-dose CAL inhibit proliferation of CFs, while high-dose CAL promotes proliferation of CFs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060520931245","type":"journal-article","created":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T06:52:15Z","timestamp":1599720735000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of parathyroid hormone-related protein on intracellular calcium ion and cyclic adenosine monophosphate concentrations in cardiac fibroblasts"],"prefix":"10.1177","volume":"48","author":[{"given":"Ping","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Second Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Children\u2019s Hospital of Harbin, Harbin City, Heilongjiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao-Ting","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Harbin No.4 Hospital, Harbin city, Heilongjiang province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Second Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang-Xia","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The Second Affiliated Hospital of Harbin Medical University,  Harbin city, Heilongjiang province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6076-0472","authenticated-orcid":false,"given":"Xuan-Yi","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Harbin No.4 Hospital, Harbin city, Heilongjiang province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,9,10]]},"reference":[{"key":"bibr1-0300060520931245","first-page":"145","volume":"18","author":"Zhou FH","year":"1998","journal-title":"Foreign Medical Sciences (Urology and Nephrology Foreign Medical 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