{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:53:55Z","timestamp":1777726435225,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"China Rheumatism and Original Plan Fund","award":["LYJH-010"],"award-info":[{"award-number":["LYJH-010"]}]},{"name":"Shanghai Special Fund for Leading Talents Construction","award":["201444"],"award-info":[{"award-number":["201444"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81671595"],"award-info":[{"award-number":["81671595"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Our objective was to explore the molecular pathogenesis of the onset of gout and the mechanism underlying the effect of interleukin (IL)-37 on PDZ domain-containing 1 (PDZK1) protein through the nuclear factor-\u03baB signaling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Real-time PCR and western blotting were used to detect expression of PDZK1 mRNA and protein, respectively, in the HK-2 cell line. The inhibitors pyrrolidine dithiocarbamate (PDTC) and wortmannin were added to HK-2 cells stimulated by IL-37, and changes in PDZK1 protein were detected by western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Based on our previous research, we used 10 \u00b5mol\/L PDTC. We detected no significant change in PDZK1 at the mRNA level among the IL-37, PDTC+IL-37, and wortmannin+IL-37 groups. With increasing IL-37 concentration, the protein level of PDZK1 increased. After adding wortmannin, the protein level of PDZK1 increased with increasing concentration of IL-37, albeit not significantly, and the level of PDZK1 remained lower than that with IL-37 alone. After adding PDTC, the protein level of PDZK1 showed a trend to decrease with increasing concentrations of IL-37 up to 40 ng\/mL. The immunofluorescence results supported the western blot results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>IL-37 can affect protein expression of PDZK1, but not at the translational level, in the pathogenesis of gout.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060520948717","type":"journal-article","created":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T15:17:31Z","timestamp":1599751051000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Interleukin-37 contributes to the pathogenesis of gout by affecting PDZ domain-containing 1 protein through the nuclear factor-kappa B pathway"],"prefix":"10.1177","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3506-3330","authenticated-orcid":false,"given":"Wei","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology, Changhai Hospital, Affiliated to Naval Military Medical University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeqing","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Changhai Hospital, Affiliated to Naval Military Medical University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianmei","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Changhai Hospital, Affiliated to Naval Military Medical University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Changhai Hospital, Affiliated to Naval Military Medical University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Changhai Hospital, Affiliated to Naval Military Medical University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3312-024X","authenticated-orcid":false,"given":"Dongbao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Changhai Hospital, Affiliated to Naval Military Medical University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,9,10]]},"reference":[{"key":"bibr1-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp030733"},{"key":"bibr2-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-011-0532-z"},{"key":"bibr3-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00650.2016"},{"key":"bibr4-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.14.10308"},{"key":"bibr5-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2011-200711"},{"key":"bibr6-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11100"},{"key":"bibr7-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-016-1167-y"},{"key":"bibr8-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.91"},{"key":"bibr9-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-019-0115-y"},{"key":"bibr10-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0b013e32835ddad2"},{"key":"bibr11-0300060520948717","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2011.00001"},{"key":"bibr12-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.460170"},{"key":"bibr13-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1944"},{"key":"bibr14-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2013.10.004"},{"key":"bibr15-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2012.11.001"},{"key":"bibr16-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111982108"},{"key":"bibr17-0300060520948717","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12284"},{"key":"bibr18-0300060520948717","first-page":"5247","volume":"8","author":"Xu 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