{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:06:21Z","timestamp":1787018781727,"version":"build-2736575974"},"reference-count":26,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the mechanism by which curcumin prevents lung injury in a rat model of limb ischaemia-reperfusion injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Rats were randomized into four groups ( n\u2009=\u200920): control group (sham group); ischaemia-reperfusion group (I\/R group); curcumin group (I\/R+Cur group); and inhibitor of agomir-21 group (I\/R+Cur+antagomir-21 group). At 3\u2009h after reperfusion, lung tissues were collected for histopathology and immunohistochemistry to determine the apoptosis index (AI). Lung injury score (LIS) and lung wet\/dry (W\/D) ratio were determined. Lung microRNA-21 (miR-21) mRNA levels were measured using reverse transcription\u2013polymerase chain reaction. Toll-like receptor 4 (TLR4) and nuclear factor kappa-B p65 (NF-\u03baB p65) protein levels were measured by Western blot analysis. Tumour necrosis factor (TNF)-\u03b1 and interleukin (IL)-1\u03b2 levels were determined by enzyme-linked immunosorbent assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the I\/R group, the W\/D, LIS, AI, miR-21 mRNA, TLR4, NF-\u03baB p65, TNF-\u03b1 and IL-1\u03b2 were significantly increased and the PaO\n                      <jats:sub>2<\/jats:sub>\n                      was decreased compared with the sham group. Evidence of lung injury was observed in the I\/R group and this was alleviated in the I\/R+Cur group. An inhibitor of miR-21 (antagomir-21) reversed the protective effects of curcumin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Curcumin post-treatment can alleviate the lung injuries induced by limb ischaemia-reperfusion via downregulating the levels of miR-21 mRNA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060520965809","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T22:46:20Z","timestamp":1606344380000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["The mechanism of curcumin post-treatment relieving lung injuries by regulating miR-21\/TLR4\/NF-\u03baB signalling pathway"],"prefix":"10.1177","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8442-8113","authenticated-orcid":false,"given":"Hai-Bo","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology, Affiliated Central Hospital, Shenyang Medical College, Shenyang, Liaoning Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Feng","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Affiliated Central Hospital, Shenyang Medical College, Shenyang, Liaoning Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,11,25]]},"reference":[{"key":"bibr1-0300060520965809","doi-asserted-by":"publisher","DOI":"10.1177\/0300060520934627"},{"key":"bibr2-0300060520965809","first-page":"1879","volume":"43","author":"Xu L","year":"2019","journal-title":"Int J Mol 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