{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:45:58Z","timestamp":1777725958398,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The present study aimed to evaluate the effects of cluster of differentiation (CD)4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      forkhead box p3 (Foxp3)\n                      <jats:sup>+<\/jats:sup>\n                      regulatory T cells (Tregs) on unexplained recurrent spontaneous abortion (URSA) and the associated mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The proportion of CD4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      Foxp3\n                      <jats:sup>+<\/jats:sup>\n                      Tregs and inflammatory cytokine concentrations in the peripheral blood of women with URSA were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. CBA\/JxDBA\/2J mating was used to establish an abortion-prone mouse model and the model mice were treated with the Toll-like receptor 4 (TLR4) antagonist E5564 and the TLR4 agonist lipopolysaccharide.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The proportion of CD4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      Foxp3\n                      <jats:sup>+<\/jats:sup>\n                      Tregs was decreased and the inflammatory response was increased in women with URSA. In the abortion-prone mouse model, E5564 significantly increased the proportion of CD4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      Foxp3\n                      <jats:sup>+<\/jats:sup>\n                      Tregs, decreased the inflammatory response, and increased Foxp3 mRNA and protein expression. Lipopolysaccharide had adverse effects on the abortion-prone model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These data suggest that CD4\n                      <jats:sup>+<\/jats:sup>\n                      CD25\n                      <jats:sup>+<\/jats:sup>\n                      Foxp3\n                      <jats:sup>+<\/jats:sup>\n                      Tregs regulate immune homeostasis in URSA via the TLR4\/nuclear factor-\u03baB pathway, and that the TLR4 antagonist E5564 may be a novel and potential drug for treating URSA.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0300060520980940","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T01:47:40Z","timestamp":1608774460000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["CD4\n                    <sup>+<\/sup>\n                    CD25\n                    <sup>+<\/sup>\n                    Foxp3\n                    <sup>+<\/sup>\n                    regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4\/nuclear factor-\u03baB pathway"],"prefix":"10.1177","volume":"48","author":[{"given":"Shuang","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China"},{"name":"Department of Gynecologic Endocrinology, Guangzhou Women and Children\u2019s Medical Center, Guangzhou, Guangdong, 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Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8988-5092","authenticated-orcid":false,"given":"Yujuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Gynecology, University of Chinese Academy of Sciences Shenzhen Hospital (Guangming), Shenzhen, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangcai","family":"Wei","sequence":"additional","affiliation":[{"name":"Guangdong Women and Children\u2019s Hospital, Guangzhou, Guangdong, 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