{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T15:59:46Z","timestamp":1779379186242,"version":"3.53.1"},"reference-count":28,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Levels of specific IgE (sIgE) against allergen components can be assessed using multiplex assays or with highly sensitive, quantitative methods. The aim of this study was to compare the sensitivity and specificity of different immunological methods for diagnosis of shrimp allergy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty patients with positive skin prick tests for frozen tiger shrimp were selected for further examination. Blood samples were taken to assess concentrations of sIgE against the house dust mites Dermatophagoides pteronyssinus and D. farinae, shrimp allergen extract, allergen components Der p 1, Der p 2 and Pan a 1 (ImmunoCap), and the ImmunoCap ISAC 112 panel.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All patients had elevated levels of sIgE against shrimp and D pteronyssinus. Eight patients were sensitized to Pen m 1, three patients were sensitized to Pen m 2, and two patients were sensitized to Pen m 4 (ISAC). ImmunoCap ISAC detected shrimp sensitization in 50% of patients. There was a strong correlation between concentrations of sIgE against Pen m1 and Der p 10 detected by ImmunoCap.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The singleplex ImmunoCap system remains the reference diagnostic method, but in the case of shrimp allergy ImmunoCap ISAC provided better insight into patient allergen profiles.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605211006597","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T03:13:38Z","timestamp":1618197218000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy"],"prefix":"10.1177","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5957-8382","authenticated-orcid":false,"given":"Natalia","family":"Ukleja-Soko\u0142owska","sequence":"first","affiliation":[{"name":"Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinga","family":"Lis","sequence":"additional","affiliation":[{"name":"Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"\u017bbikowska-Gotz","sequence":"additional","affiliation":[{"name":"Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafa\u0142","family":"Adamczak","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Ku\u017ami\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zbigniew","family":"Bartuzi","sequence":"additional","affiliation":[{"name":"Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,4,12]]},"reference":[{"key":"bibr1-03000605211006597","unstructured":"Warsaw Medical University. Epidemiologia chor\u00f3b alergicznych w Polsce, http:\/\/ecap.pl\/pdf\/ECAP_wyniki_pl.pdf (2020, accessed 23 March 2021)."},{"key":"bibr2-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2010.11.014"},{"key":"bibr3-03000605211006597","first-page":"1","volume":"13","author":"Rosenfield L","year":"2017","journal-title":"Ann Allergy Asthma Immunol"},{"key":"bibr4-03000605211006597","doi-asserted-by":"publisher","DOI":"10.23822\/EurAnnACI.1764-1489.108"},{"key":"bibr5-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.06.038"},{"key":"bibr6-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-010-0149-4"},{"key":"bibr7-03000605211006597","unstructured":"Macro Array Diagnostics. ALEX2\u00ae allergy explorer \u2013 the smart way to explore allergy, https:\/\/www.macroarraydx.com\/alex (2020, accessed 23 March 2021)."},{"key":"bibr8-03000605211006597","unstructured":"Thermo Fisher Scientific. ImmunoCAP ISAC E112i, http:\/\/www.thermofisher.com\/phadia\/wo\/en\/product-catalog.html?articleNumber=81-1020-01 (2020, accessed 23 March 2021)."},{"key":"bibr9-03000605211006597","unstructured":"CAAM \u2013 Centri Associati Di Allergologia Molecolare. FABER, the screening test for IgE-mediated allergies: A precise, comprehensive, unique, and powerful tool, https:\/\/www.caam-allergy.com\/en\/faber (2020, accessed 23 March 2021)."},{"key":"bibr10-03000605211006597","first-page":"221","volume":"23","author":"Ukleja-Soko\u0142owska N","year":"2018","journal-title":"Alerg Astma Immun"},{"key":"bibr11-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-020-0902-2"},{"key":"bibr12-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1159\/000324470"},{"key":"bibr13-03000605211006597","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.9.5470"},{"key":"bibr14-03000605211006597","first-page":"20","volume":"42","author":"Villalta D","year":"2010","journal-title":"Eur Ann Allergy Clin Immunol"},{"key":"bibr15-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1111\/all.13776"},{"key":"bibr16-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2008.02048.x"},{"key":"bibr17-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(98)00006-2"},{"key":"bibr18-03000605211006597","first-page":"240","volume":"13","author":"Yadzir ZH","year":"2014","journal-title":"Iran J Allergy Asthma Immunol"},{"key":"bibr19-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1186\/s13601-018-0207-x"},{"key":"bibr20-03000605211006597","first-page":"172","volume":"46","author":"Giuffrida MG","year":"2014","journal-title":"Eur Ann Allergy Clin Immunol"},{"key":"bibr21-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2014.09.011"},{"key":"bibr22-03000605211006597","first-page":"48","volume":"68","author":"Tonomura K","year":"2019","journal-title":"Arerugi"},{"key":"bibr23-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.13890"},{"key":"bibr24-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2019.05.006"},{"key":"bibr25-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1177\/2058738420907188"},{"key":"bibr26-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1159\/000464326"},{"key":"bibr27-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1007\/s40629-015-0046-4"},{"key":"bibr28-03000605211006597","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.07.010"}],"container-title":["Journal of International Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03000605211006597","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03000605211006597","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03000605211006597","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:48:51Z","timestamp":1777459731000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03000605211006597"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1177\/03000605211006597"],"URL":"https:\/\/doi.org\/10.1177\/03000605211006597","relation":{},"ISSN":["0300-0605","1473-2300"],"issn-type":[{"value":"0300-0605","type":"print"},{"value":"1473-2300","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4]]},"article-number":"03000605211006597"}}