{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T13:09:54Z","timestamp":1777727394723,"version":"3.51.4"},"reference-count":35,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Ningxia Hui Autonomous Region key research and development plan project","award":["2019BFG02005"],"award-info":[{"award-number":["2019BFG02005"]}]},{"name":"2018 Advantageous Subjects Project of Ning Xia Medical University","award":["XY201807"],"award-info":[{"award-number":["XY201807"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960277"],"award-info":[{"award-number":["81960277"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"First-Class Discipline Construction Founded Project of Ning Xia Medical University and the School of Clinical Medicine","award":["NXYLXK2017A05"],"award-info":[{"award-number":["NXYLXK2017A05"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We aimed to identify the factors that influence serum anti-M\u00fcllerian hormone (AMH) concentration measurements.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We collected serum samples between May and September 2018 and compared the effect on AMH concentration measured by ELISA of conditions including venepuncture, storage time, storage temperature, locations of the reaction microplate, and the use of the oral contraceptive pill and gonadotrophin-releasing hormone (GnRH).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>AMH concentration was not affected by food intake but was affected by haemolysis. It was also much higher in samples on the edge of the ELISA microtitre plate. AMH concentration increased after incubation at room temperature for 1 day, 4\u00b0C for 3 days, \u221220\u00b0C for 1 month and \u221240\u00b0C for 4 months, but no change occurred during storage at \u221280\u00b0C for 9 months. AMH concentration was high in patients following GnRH agonist treatment but was not affected by oral contraceptives.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>No fasting is required prior to AMH measurement. Placement of serum samples on the edge of microtitre plates affects the results of the AMH ELISA. If serum samples cannot be assayed immediately, it is best to store them at \u221280\u00b0C. Basal AMH concentration cannot be used as a measure of ovarian reserve after GnRH agonist treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605211016161","type":"journal-article","created":{"date-parts":[[2021,5,29]],"date-time":"2021-05-29T16:06:18Z","timestamp":1622304378000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Factors affecting the accuracy and reliability of the measurement of anti-M\u00fcllerian hormone concentration in the clinic"],"prefix":"10.1177","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4372-1433","authenticated-orcid":false,"given":"Yun-Xing","family":"Fu","sequence":"first","affiliation":[{"name":"Ningxia Medical University, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 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