{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:06:21Z","timestamp":1787018781934,"version":"build-2736575974"},"reference-count":36,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Myasthenia gravis (MG) is a chronic autoimmune neuromuscular disorder. Recent studies report that long non-coding RNAs (lncRNAs) play vital roles in the pathogenesis of various diseases. This study explored the molecular mechanism of lncRNA growth arrest specific 5 (GAS5) in regulating the T helper 17 (Th17)\/regulatory T (Treg) cell balance in MG.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      GAS5 and miR-23a expression levels were detected by quantitative reverse transcription polymerase chain reaction. Flow cytometry was performed to examine the proportion of Th17 and Treg cells in CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells from MG patients. The interaction between GAS5 and miR-23a was verified by luciferase reporter and RNA immunoprecipitation assays. Levels of Th17 and Treg-related proteins were examined using western blots and enzyme-linked immunosorbent assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      GAS5 expression levels were significantly decreased in the CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells of MG patients, and GAS5 overexpression restrained Th17 differentiation in CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells. Moreover, miR-23a was confirmed as a downstream target of GAS5 and negatively regulated by GAS5 through a direct interaction. Further exploration showed that GAS5 can inhibit Th17 differentiation by downregulating miR-23a.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Collectively, our results indicate that GAS5 can regulate the Th17\/Treg balance by targeting miR-23a expression, providing a scientific basis for clinical therapeutic development for MG.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605211053703","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T02:37:05Z","timestamp":1655347025000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Long non-coding RNA growth arrest specific 5 regulates the T helper 17\/regulatory T balance by targeting miR-23a in myasthenia gravis"],"prefix":"10.1177","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6957-3825","authenticated-orcid":false,"given":"Yingying","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou City, Fujian Province, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqun","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Emergency, The Second Affiliated Hospital of Fujian Medical University, Quanzhou City, Fujian Province, P. R. 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