{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T11:04:25Z","timestamp":1794049465439,"version":"4.8.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To analyse macrolide resistance and molecular characteristics of Bordetella pertussis clinical isolates from western China, and to explore the relationship between macrolide-resistance and genotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Susceptibilities of B. pertussis clinical isolates to erythromycin, azithromycin and clarithromycin were determined by epsilometer test (E-test). Isolated strains were sequenced to ascertain the presence of the 23S rRNA gene A2047G mutation. Strains were typed using multilocus antigen sequence typing, multilocus variable-number tandem-repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 58 B. pertussis strains isolated in this study, 46 were macrolide-resistant and 12 were macrolide sensitive. All macrolide-resistant strains carried the A2047G mutation and were the prn1\/ ptxP1\/ ptxA1\/ fim3-1\/ fim2-1 genotype; the MLVA types were MT195 (19\/58), MT55 (13\/58) and MT104 (14\/58), and the PFGE profiles were classified into BpSR23 (17\/58) and BpFINR9 (29\/58) types. None of the macrolide-sensitive strains carried the A2047G mutation; genotypes were ( prn9 or prn2)\/ ptxP3\/ ptxA1\/ fim3-1\/ fim2-1, and all were MT27. PFGE profiles differed from the macrolide-resistant strains.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>B. pertussis clinical isolates from western China were severely resistant to macrolides. Genotypes differed between macrolide-resistant and macrolide-sensitive strains, and there may be a correlation between acquisition of macrolide resistance and changes in specific molecular types.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605221078782","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T11:33:25Z","timestamp":1646048005000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Macrolide susceptibility and molecular characteristics of\n                    <i>Bordetella pertussis<\/i>"],"prefix":"10.1177","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6236-1520","authenticated-orcid":false,"given":"Juansheng","family":"Zhang","sequence":"first","affiliation":[{"name":"Microbiology laboratory, Xi\u2019an Centre for Disease Control and Prevention, Xi'an, Shaanxi Province, 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