{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:37:35Z","timestamp":1792640255648,"version":"4.5.0"},"reference-count":33,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study was performed to determine the effect of swim-up (SU) and density gradient centrifugation (DGC) on sperm survival and DNA fragmentation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Individual semen samples were analyzed before each was divided into two aliquots (half for SU and half for DGC) for calculation of sperm survival and the DNA fragmentation index (DFI). Sperm DNA fragmentation was determined using the sperm chromatin dispersion test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The DFI of the 63 semen samples processed using both procedures was lower than that of the fresh semen samples. The DFI was significantly lower for samples processed using the SU than DGC method. In the sperm survival test, the SU technique was associated with increased sperm motility and vitality following preparation. After 24 hours, however, the concentration and percentage of surviving sperm were significantly lower in the SU than DGC group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Both semen preparation techniques help to minimize sperm DNA fragmentation; however, when the DFI is &lt;30%, the SU technique is more appropriate than DGC. While DGC may be superior for intrauterine insemination, the SU method may be preferable for in vitro fertilization or maturation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605221097492","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T17:39:36Z","timestamp":1652895576000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of sperm preparation techniques on sperm survivability and DNA fragmentation"],"prefix":"10.1177","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6225-3108","authenticated-orcid":false,"given":"Minh Tam","family":"Le","sequence":"first","affiliation":[{"name":"Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Vietnam"},{"name":"Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Nhan Thi","family":"Dang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trung Van","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thai Thanh Thi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quoc Huy Vu","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngoc Thanh","family":"Cao","sequence":"additional","affiliation":[{"name":"Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Vietnam"},{"name":"Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, 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