{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T04:07:10Z","timestamp":1792814830044,"version":"4.5.0"},"reference-count":50,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Research platform for Mechanism and Diagnosis of Pathogens of Respiratory Tract in Children","award":["SEY-GSP-YXPT-A02"],"award-info":[{"award-number":["SEY-GSP-YXPT-A02"]}]},{"name":"Pathogen High-Throughput Gene Sequencing Technology Engineering Laboratory of Shenzhen","award":["Deepafai (2019) 986"],"award-info":[{"award-number":["Deepafai (2019) 986"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Respiratory syncytial virus (RSV) and respiratory adenovirus (ADV) are two common pathogens that cause acute respiratory tract infections in children. We aimed to develop a rapid method for detecting both pathogens simultaneously.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The recombinase polymerase isothermal amplification (RPA) method was combined with the CRISPR\/Cas detection system. The assay\u2019s specificity and sensitivity were explored by designing RPA primers and CRISPR RNAs (crRNAs) through multi-sequence comparisons, optimizing the reaction conditions, and using a fluorescent reading device. The consistency of the test results of 160 clinical pharyngeal swab samples was studied using quantitative polymerase chain reaction (qPCR) results as a comparative control.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      RSV and ADV could be detected at levels as low as 10\n                      <jats:sup>4<\/jats:sup>\n                      copies\/mL and 10\n                      <jats:sup>3<\/jats:sup>\n                      copies\/mL, respectively, within 50 minutes with no cross-reactivity with other similar pathogens. For the clinical samples, compared with the qPCR method, the sensitivities for RSV and ADV were 98.1% and 91.4%, respectively, and the detection specificities were both 100%. The Kappa values were greater than 0.95, suggesting a high degree of consistency.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This method for detecting RSV and ADV is rapid, sensitive, and specific. It can accurately detect mixed infections in a timely manner, making it suitable for use in areas with scarce healthcare resources.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605231223083","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T06:36:40Z","timestamp":1705473400000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["A multiplex recombinase polymerase amplification assay combined with CRISPR\/Cas12a for the detection of respiratory syncytial virus and respiratory adenovirus"],"prefix":"10.1177","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8866-8846","authenticated-orcid":false,"given":"Gao","family":"Hongdan","sequence":"first","affiliation":[{"name":"School of Laboratory Medicine, Bengbu Medical College, Bengbu, Anhui, China"},{"name":"Institute of Pediatrics, Shenzhen Children\u2019s Hospital, Shenzhen, Guangdong, 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