{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:31:09Z","timestamp":1793662269213,"version":"4.5.1"},"reference-count":35,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"The Traditional Chinese Medicine Project of Zhejiang Province","award":["2022ZB396"],"award-info":[{"award-number":["2022ZB396"]}]},{"name":"The Medical and Health Research Project of Zhejiang Province","award":["2024KY1799"],"award-info":[{"award-number":["2024KY1799"]}]},{"name":"The Science and Technology Project of Taizhou City","award":["XM20220596"],"award-info":[{"award-number":["XM20220596"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the possible correlation between histone deacetylase inhibition and radiosensitivity in PC-3 prostate cancer cells and to explore the possible mechanism involved.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>PC-3 prostate cancer cells were treated with 0.40\u2009\u03bcM CG-745 alone, radiation alone, or 0.40\u2009\u03bcM CG-745 in combination with radiation. A CCK-8 kit was used to measure the proliferation of PC-3 cells. The colony formation assay was used to determine cell reproductive survival. Immunofluorescence analysis was used to detect the location of phosphorylated H2AX foci. Apoptosis and cell cycle distribution of PC-3 cells were assessed via flow cytometry analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CG-745 enhances the repressive effect of irradiation on PC-3 cell growth, as shown by the CCK-8 assay and colony formation assay. Compared with CG-745 or radiation alone, CG-745 combined with radiation significantly increased phosphorylated H2AX foci formation. The combination of CG-745 and radiation significantly increased the percentage of cells in the S phase and decreased percentage of cells in the G2 and G1 phases compared with treatment with CG-745 alone or radiation alone. Flow cytometry analysis showed that CG-745 promoted the PC-3 cell apoptosis induced by radiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The histone deacetylase inhibitor CG-745 enhanced radiation-induced DNA damage, cell cycle arrest, and cell apoptosis, thus increasing the radiosensitivity of PC-3 prostate cancer cells to X-ray irradiation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605251371562","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T12:50:39Z","timestamp":1756817439000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["The histone deacetylase inhibitor CG-745 enhances the radiosensitivity of prostate cancer cells"],"prefix":"10.1177","volume":"53","author":[{"given":"Bin","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Mengchao Hepatobiliary Hospital of Fujian Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6566-3572","authenticated-orcid":false,"given":"Shao-Guang","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Mengchao Hepatobiliary Hospital of Fujian Medical University, China"},{"name":"Department of Radiation Oncology, Huangyan Hospital of Wenzhou Medical University, Taizhou First People\u2019s Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20021321"},{"key":"e_1_3_8_3_2","first-page":"166","article-title":"Histone deacetylase inhibitors: molecular mechanisms of action and clinical trials as anti-cancer drugs","volume":"3","author":"Kim HJ","year":"2011","unstructured":"Kim HJ, Bae SC. Histone deacetylase inhibitors: molecular mechanisms of action and clinical trials as anti-cancer drugs. 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